The Identification of Six Novel Proteins with Fibronectin or Collagen Type І Binding Activity from Streptococcus suis Serotype 2

Hui Zhang; Junxi Zheng; Li Yi; Yue Li; Zhe Ma; Hongjie Fan; Chengping Lu

doi:10.1007/s12275-014-4311-x

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The Identification of Six Novel Proteins with Fibronectin or Collagen Type І Binding Activity from Streptococcus suis Serotype 2: Hui Zhang ^1,2, Junxi Zheng ¹, Li Yi ¹, Yue Li ¹, Zhe Ma ¹, Hongjie Fan ^1,3, Chengping Lu ^1,3; Journal of Microbiology 2014;52(11):963-969.
DOI: https://doi.org/10.1007/s12275-014-4311-x
Published online: October 31, 2014

¹College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, P. R. China, ²China Animal Health and Epidemiology Center, Qingdao, P. R. China, ³Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, P. R. China¹College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, P. R. China, ²China Animal Health and Epidemiology Center, Qingdao, P. R. China, ³Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, P. R. China

Corresponding author: Hongjie Fan , Tel: +86-025-84395605,

Received: 27 May 2014 • Revised: 11 September 2014 • Accepted: 12 September 2014

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Abstract

Streptococcus suis, a major swine pathogen, is an emerging zoonotic agent that causes meningitis and septic shock. Bacterial cell wall and secreted proteins are often involved in interactions with extracellular matrix proteins (ECMs), which play important roles in the initial steps of pathogenesis. In this study, 2D SDS-PAGE, western blotting-based binding affinity measurements, and microtiter plate binding assays were used to identify cell wall and secreted proteins from S. suis that interact with fibronectin and collagen type І. We identified six proteins from S. suis, including three proteins (translation elongation factor G, oligopeptide-binding protein OppA precursor, and phosphoglycerate mutase) that show both fibronectin and collagen type І binding activity. To the best of our knowledge, these three newly identified proteins had no previously reported fibronectin or collagen type І binding activity. Overall, the aim in this study was to identify proteins with ECM binding activity from S. suis and it represents the first report of six new proteins from S. suis that interact with fibronectin or collagen type І.

Keywords: Streptococcus suis;cell wall proteins;secreted proteins;extracellular matrix protein

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The Identification of Six Novel Proteins with Fibronectin or Collagen Type І Binding Activity from Streptococcus suis Serotype 2

J. Microbiol. 2014;52(11):963-969. Published online October 31, 2014

DOI: https://doi.org/10.1007/s12275-014-4311-x

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