Abstract

A Gram-negative, yellow-pigmented, long-rod shaped bacterial strain designated DY10T was isolated from a soil sample collected at Mt. Deogyusan, Jeonbuk province, South Korea. Optimum growth observed at 30°C and pH 7. No growth was observed above 1% (w/v) NaCl. Comparative 16S rRNA gene sequence analysis showed that strain DY10T belonged to the genus Spirosoma and was distantly related to Spirosoma arcticum R2-35T (91.0%), Spirosoma lingual DSM 74T (90.8%), Spirosoma endophyticum EX36T (90.7%), Spirosoma panaciterrae DSM 21099T (90.5%), Spirosoma rigui WPCB118T (90.2%), Spirosoma spitsbergense DSM 19989T (89.8%), Spirosoma luteum DSM 19990T (89.6%), Spirosoma oryzae RHs22T (89.6%), and Spirosoma radiotolerans DG5AT (89.1%). Strain DY10T showed resistance to gamma and ultraviolet radiation. The chemotaxonomic characteristics of strain DY10T were consistent with those of the genus Spirosoma, with the quinone system with MK-7 as the predominant menaquinone, iso-C15:0, C16:1 ω5c, and summed feature3 (C16:1 ω7c/C16:1 ω6c), and phosphatidylethanolamine as the major polar lipid. The G+C content of the genomic DNA was 53.0 mol%. Differential phenotypic properties with the closely related type strains clearly distinguished strain DY10T from previously described members of the genus Spirosoma and represents a novel species in this genus, for which the name Spirosoma montaniterrae sp. nov. is proposed. The type strain is DY10T (=KCTC 23999T =KEMB 9004-162T =JCM 18492T).

• Keywords: Cytophagaceae; Spirosoma montaniterrae; radiation-resistant; taxonomy