Pregnancy - associated human listeriosis: Virulence and genotypic analysis of Listeria monocytogenes from clinical samples

Dharmendra Kumar Soni; Durg Vijai Singh; Suresh Kumar Dubey

doi:10.1007/s12275-015-5243-9

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Pregnancy - associated human listeriosis: Virulence and genotypic analysis of Listeria monocytogenes from clinical samples: Dharmendra Kumar Soni ¹, Durg Vijai Singh ², Suresh Kumar Dubey ¹; Journal of Microbiology 2015;53(9):653-660.
DOI: https://doi.org/10.1007/s12275-015-5243-9
Published online: August 1, 2015

¹Environmental Microbiology Laboratory, Department of Botany, Banaras Hindu University, Varanasi-221005, India, ²Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar-751023, India¹Environmental Microbiology Laboratory, Department of Botany, Banaras Hindu University, Varanasi-221005, India, ²Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar-751023, India

Corresponding author: Suresh Kumar Dubey , Tel: +91-542-2307147,

Received: 12 May 2015 • Revised: 26 June 2015 • Accepted: 15 July 2015

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Abstract

Listeria monocytogenes, a life-threatening pathogen, poses severe risk during pregnancy, may cause abortion, fetal death or neonatal morbidity in terms of septicemia and meningitis. The present study aimed at characterizing L. monocytogenes isolated from pregnant women based on serotyping, antibiotic susceptibility, virulence genes, in vivo pathogenicity test and ERIC- and REP-PCR fingerprint analyses. The results revealed that out of 3700 human clinical samples, a total of 30 (0.81%) isolates [12 (0.80%) from placental bit (1500), 18 (0.81%) from vaginal swab (2200)] were positive for L. monocytogenes. All the isolates belonged to serogroup 4b, and were + ve for virulence genes tested i.e. inlA, inlC, inlJ, plcA, prfA, actA, hlyA, and iap. Based on the mice inoculation tests, 20 isolates showed 100% and 4 isolates 60% relative virulence while 6 isolates were non-pathogenic. Moreover, 2 and 10 isolates were resistant to ciprofloxacin and cefoxitin, respectively, while the rest susceptible to other antibiotics used in this study. ERIC- and REP-PCR collectively depicted that the isolates from placental bit and vaginal swab had distinct PCR fingerprints except a few isolates with identical patterns. This study demonstrates prevalence of pathogenic strains mostly resistant to cefoxitin and/or ciprofloxacin. The results indicate the importance of isolating and characterizing the pathogen from human clinical samples as the pre-requisite for accurate epidemiological investigations.

Keywords: antibiotic susceptibility;serotype identification;virulence genes;mice virulence assay;ERIC- and REP-PCR

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Pregnancy - associated human listeriosis: Virulence and genotypic analysis of Listeria monocytogenes from clinical samples

J. Microbiol. 2015;53(9):653-660. Published online August 1, 2015

DOI: https://doi.org/10.1007/s12275-015-5243-9

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