GPH1 is involved in glycerol accumulation in the three-dimensional networks of the nematode-trapping fungus Arthrobotrys oligospora

Qin-Yi Wu; Yue-Yan Zhu; Cheng-Gang Zou; Ying-Qian Kang; Lian-Ming Liang

doi:10.1007/s12275-016-6272-8

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GPH1 is involved in glycerol accumulation in the three-dimensional networks of the nematode-trapping fungus Arthrobotrys oligospora: Qin-Yi Wu ¹, Yue-Yan Zhu ¹, Cheng-Gang Zou ¹, Ying-Qian Kang ², Lian-Ming Liang ¹; Journal of Microbiology 2016;54(11):768-773.
DOI: https://doi.org/10.1007/s12275-016-6272-8
Published online: October 29, 2016

¹State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, P. R. China, ²Department of Microbiology, Guizhou Medical University, Guiyang 550004, P. R. China¹State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, P. R. China, ²Department of Microbiology, Guizhou Medical University, Guiyang 550004, P. R. China

Corresponding author: Ying-Qian Kang , Tel: -,
Lian-Ming Liang , Tel: -,

Received: 9 June 2016 • Revised: 8 September 2016 • Accepted: 13 September 2016

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Abstract

Turgor is very important for the invasive growth of fungal pathogens. Glycerol, a highly osmotic solvent, is considered to play an important role in turgor generation. The nematophagous fungus Arthrobotrys oligospora mainly lives as a saprophyte. In the presence of nematodes, A. oligospora enters the parasitic stage by forming three-dimensional networks (traps) to capture nematodes. In A. oligospora, we found that glycerol accumulated during nematode-induced trap formation. We demonstrated that deleting gph1, which encodes glycogen phosphorylase, decreased the glycerol content, compared with that of a wild-type strain. Although the number of traps induced by nematodes was not affected in the Δgph1 mutant, the capture rate was lower. Meanwhile, deleting gph1 also affected the growth rate and conidiation capacity of the fungus. These results indicate that glycerol derived from GPH1 is essential for the full virulence of A. oligospora against nematodes.

Keywords: glycerol generation;glycogen phosphorylase;nematode-trapping fungi;trap formation

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GPH1 is involved in glycerol accumulation in the three-dimensional networks of the nematode-trapping fungus Arthrobotrys oligospora

J. Microbiol. 2016;54(11):768-773. Published online October 29, 2016

DOI: https://doi.org/10.1007/s12275-016-6272-8

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