Exploring the oral microflora of preschool children

Wen Ren; Qun Zhang; Xuenan Liu; Shuguo Zheng; Lili Ma; Feng Chen; Tao Xu; Baohua Xu

doi:10.1007/s12275-017-6474-8

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Exploring the oral microflora of preschool children: Wen Ren ¹, Qun Zhang ¹, Xuenan Liu ¹, Shuguo Zheng ¹, Lili Ma ², Feng Chen ³, Tao Xu ¹, Baohua Xu ²; Journal of Microbiology 2017;55(7):531-537.
DOI: https://doi.org/10.1007/s12275-017-6474-8
Published online: April 22, 2017

¹Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, P. R. China, ²Stomatology Center, China-Japan Friendship Hospital, Beijing 100029, P. R. China, ³Central Laboratory, Peking University School and Hospital of Stomatology, Beijing 100081, P. R. China¹Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, P. R. China, ²Stomatology Center, China-Japan Friendship Hospital, Beijing 100029, P. R. China, ³Central Laboratory, Peking University School and Hospital of Stomatology, Beijing 100081, P. R. China

Corresponding author: Tao Xu , Tel: +86-010-84205268,
Baohua Xu , Tel: +86-010-84205268,

Received: 21 September 2016 • Revised: 3 March 2017 • Accepted: 6 March 2017

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Abstract

The oral cavity is one of the most important and complicated habitats in our body and supports diverse microbial communities. In this study, we aimed to determine the bacterial diversity and composition of various oral micro-niches. Samples were collected from supragingival plaque, saliva, and tongue coating from 10 preschool children (30 samples total). 16S rRNA gene pyrosequencing dataset generated 314,639 clean reads with an average of 10,488 ± 2,787 reads per sample. The phyla Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, and Fusobacteria were predominant, accounting for more than 90% of the total sequences. We found the highest α diversity, microbial richness, and evenness in plaque, compared with saliva and tongue coating. Plaque was also distinguished from saliva and tongue coating by phylogenetic distances (weighted UniFrac). Taxa with different relative abundances were further identified, confirming the existence of microbial differences across the three niches. Core microbiomes were defined of each niche; however, only a small proportion of operational taxonomic units (8.07%) were shared by the three niches. Coaggregation between Actinomyces spp. and Streptococcus spp. and other correlations among periodontal pathogens, such as Prevotella, Fusobacteria, Capnocytophaga, and Tannerella, were shown by a co-occurrence network. In summary, our study provides a framework of oral microbial communities in the population of preschool children as a baseline for further studies of oral diseases related to microbes.

Keywords: 16S rRNA gene sequencing;PICRUSt;microbiome;supragingival plaque;saliva;tongue coating

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Exploring the oral microflora of preschool children

J. Microbiol. 2017;55(7):531-537. Published online April 22, 2017

DOI: https://doi.org/10.1007/s12275-017-6474-8

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