Isolation, cultivation, and genome analysis of proteorhodopsincontaining SAR116-clade strain Candidatus Puniceispirillum marinum IMCC1322

Junhak Lee; Kae Kyoung Kwon; Seung-Il Lim; Jaeho Song; Ah Reum Choi; Sung-Hyun Yang; Kwang-Hwan Jung; Jung-Hyun Lee; Sung Gyun Kang; Hyun-Myung Oh; Jang-Cheon Cho

doi:10.1007/s12275-019-9001-2

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Isolation, cultivation, and genome analysis of proteorhodopsincontaining SAR116-clade strain Candidatus Puniceispirillum marinum IMCC1322: Junhak Lee ^1,2, Kae Kyoung Kwon ³, Seung-Il Lim ⁴, Jaeho Song ⁵, Ah Reum Choi ⁶, Sung-Hyun Yang ³, Kwang-Hwan Jung ⁶, Jung-Hyun Lee ³, Sung Gyun Kang ³, Hyun-Myung Oh ¹, Jang-Cheon Cho ⁵; Journal of Microbiology 2019;57(8):676-687.
DOI: https://doi.org/10.1007/s12275-019-9001-2
Published online: June 14, 2019

¹Institute of Liberal Arts Education, Pukyong National University, Busan 48547, Republic of Korea, ²Korea Marine Equipment Research Institute, Busan 46754, Republic of Korea, ³Marine Biotechnology Research Division, Korea Institute of Ocean Science & Technology, Busan 49111, Republic of Korea, ⁴Cell Biotech, Co., Ltd., Gimpo 10003, Republic of Korea, ⁵Division of Biology and Ocean Sciences, Inha University, Incheon 22212, Republic of Korea, ⁶Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul 04107, Republic of Korea¹Institute of Liberal Arts Education, Pukyong National University, Busan 48547, Republic of Korea, ²Korea Marine Equipment Research Institute, Busan 46754, Republic of Korea, ³Marine Biotechnology Research Division, Korea Institute of Ocean Science & Technology, Busan 49111, Republic of Korea, ⁴Cell Biotech, Co., Ltd., Gimpo 10003, Republic of Korea, ⁵Division of Biology and Ocean Sciences, Inha University, Incheon 22212, Republic of Korea, ⁶Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul 04107, Republic of Korea

Corresponding author: Hyun-Myung Oh , Tel: +82-51-629-6869,
Jang-Cheon Cho , Tel: +82-51-629-6869,

Received: 2 January 2019 • Revised: 11 April 2019 • Accepted: 16 April 2019

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Abstract

Strain IMCC1322 was isolated from a surface water sample from the East Sea of Korea. Based on 16S rRNA analysis, IMCC1322 was found to belong to the OCS28 sub-clade of SAR116. The cells appeared as short vibrioids in logarithmicphase culture, and elongated spirals during incubation with mitomycin or in aged culture. Growth characteristics of strain IMCC1322 were further evaluated based on genomic information; proteorhodopsin (PR), carbon monoxide dehydrogenase, and dimethylsulfoniopropionate (DMSP)-utilizing enzymes. IMCC1322 PR was characterized as a functional retinylidene protein that acts as a light-driven proton pump in the cytoplasmic membrane. However, the PR-dependent phototrophic potential of strain IMCC1322 was only observed under CO-inhibited and nutrient-limited culture conditions. A DMSP-enhanced growth response was observed in addition to cultures grown on C1 compounds like methanol, formate, and methane sulfonate. Strain IMCC1322 cultivation analysis revealed biogeochemical processes characteristic of the SAR116 group, a dominant member of the microbial community in euphotic regions of the ocean. The polyphasic taxonomy of strain IMCC1322 is given as Candidatus Puniceispirillum marinum, and was confirmed by chemotaxonomic tests, in addition to 16S rRNA phylogeny and cultivation analyses.

Keywords: Canididatus Puniceispirillum marinum;carbon monoxide;dimethylsulfoniopropionate;light-emitting diode;mitomycin C;proteorhodopsin;SAR116

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Isolation, cultivation, and genome analysis of proteorhodopsincontaining SAR116-clade strain Candidatus Puniceispirillum marinum IMCC1322

J. Microbiol. 2019;57(8):676-687. Published online June 14, 2019

DOI: https://doi.org/10.1007/s12275-019-9001-2

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