Vibrio parahaemolyticus cqsA controls production of quorum sensing signal molecule 3-hydroxyundecan-4-one and regulatessensing signal molecule 3-hydroxyundecan-4-one and regulates colony morphology

Kui Wu; Yangyun Zheng; Qingping Wu; Haiying Chen; Songzhe Fu; Biao Kan; Yongyan Long; Xiansheng Ni; Junling Tu

doi:10.1007/s12275-019-9379-x

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Vibrio parahaemolyticus cqsA controls production of quorum sensing signal molecule 3-hydroxyundecan-4-one and regulatessensing signal molecule 3-hydroxyundecan-4-one and regulates colony morphology: Kui Wu ^1,2,4, Yangyun Zheng ¹, Qingping Wu ², Haiying Chen ¹, Songzhe Fu ³, Biao Kan ⁴, Yongyan Long ^1,4, Xiansheng Ni ¹, Junling Tu ¹; Journal of Microbiology 2019;57(12):1105-1114.
DOI: https://doi.org/10.1007/s12275-019-9379-x
Published online: November 4, 2019

¹The Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control, Nanchang Center for Disease Control and Prevention, Nanchang, 330038, P. R. China, ²State Key Laboratory of Applied Microbiology South China, Guangdong, Institute of Microbiology, Guangzhou, 510070, P. R. China, ³College of Marine Technology and Environment, Dalian Ocean University, Dalian, 116023, P. R. China, ⁴State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, P. R. China¹The Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control, Nanchang Center for Disease Control and Prevention, Nanchang, 330038, P. R. China, ²State Key Laboratory of Applied Microbiology South China, Guangdong, Institute of Microbiology, Guangzhou, 510070, P. R. China, ³College of Marine Technology and Environment, Dalian Ocean University, Dalian, 116023, P. R. China, ⁴State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, P. R. China

Corresponding author: Kui Wu , Tel: +86-791863632255,
Qingping Wu , Tel: +86-791863632255,

Received: 5 August 2019 • Revised: 24 September 2019 • Accepted: 24 September 2019

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Abstract

In order to adapt to different environments, Vibrio parahaemolyticus employed a complicated quorum sensing system to orchestrate gene expression and diverse colony morphology patterns. In this study, the function of the putative quorum sensing signal synthase gene cqsA (VPA0711 in V. parahaemolyticus strain RIMD2210633 genome) was investigated. The cloning and expression of V. parahaemolyticus cqsA in Escherichia coli system induced the production of a new quorum sensing signal that was found in its culture supernatant. The signal was purified by high performance liquid chromatography
methods
and determined to be 3-hydroxyundecan- 4-one by indirect and direct mass spectra assays. The deletion of cqsA in RIMD2210633 changed V. parahaemolyticus colony morphology from the classical ‘fried-egg’ shape (thick and opaque in the center, while thin and translucent in the edge) of the wild-type colony to a ‘pancake’ shape (no significant difference between the centre and the edge) of the cqsAdeleted colony. This morphological change could be restored by complementary experiment with cqsA gene or the signal extract. In addition, the expression of opaR, a well-known quorum sensing regulatory gene, could be up-regulated by cqsA deletion. Our results suggested that V. parahaemolyticus used cqsA to produce 3-hydroxyundecan-4-one signal and thereby regulated colony morphology and other quorum sensing-associated behaviors.

Keywords: Vibrio parahaemolyticus;quorum sensing;cqsA;3-hydroxyundecan-4-one;colony morphology

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Vibrio parahaemolyticus cqsA controls production of quorum sensing signal molecule 3-hydroxyundecan-4-one and regulatessensing signal molecule 3-hydroxyundecan-4-one and regulates colony morphology

J. Microbiol. 2019;57(12):1105-1114. Published online November 4, 2019

DOI: https://doi.org/10.1007/s12275-019-9379-x

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