The effect of the HRB linker of Newcastle disease virus fusion protein on the fusogenic activity

Yaqing Liu; Ying Liu; Yanan Huang; Hongling Wen; Li Zhao; Yanyan Song; Zhiyu Wang

doi:10.1007/s12275-021-0539-4

Articles

Page Path: HOME > J. Microbiol > Volume 59(5); 2021 > Article

Journal Article
The effect of the HRB linker of Newcastle disease virus fusion protein on the fusogenic activity: Yaqing Liu ¹, Ying Liu ², Yanan Huang ¹, Hongling Wen ¹, Li Zhao ¹, Yanyan Song ¹, Zhiyu Wang ¹; Journal of Microbiology 2021;59(5):513-521.
DOI: https://doi.org/10.1007/s12275-021-0539-4
Published online: March 29, 2021

¹Department of Virology, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan 250014, P. R. China, ²Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250014, P. R. China¹Department of Virology, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan 250014, P. R. China, ²Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250014, P. R. China

Corresponding author: Zhiyu Wang , Tel: +86-531-88382625,

Received: 21 October 2020 • Revised: 5 February 2021 • Accepted: 5 February 2021

prev next

73 Views
0 Download
5 Web of Science
5 Crossref
5 Scopus

Full Article

Abstract

Newcastle disease, designated a class A disease of poultry by the Office international des epizooties (OIE), is an acute infection caused by Newcastle disease virus (NDV). The merging of the envelope of NDV with the membrane of a target host cell is the key step in the infection pathway, which is driven by the concerted action of two glycoproteins: haemagglutinin- neuraminidase (HN) and fusion (F) protein. When the HN protein binds to the host cell surface receptor, the F protein is activated to mediate fusion. The three-dimensional structure of the F protein has been reported to have low electron density between the DIII domain and the HRB domain, and this electron-poor region is defined as the HRB linker. To clarify the contributing role of the HRB linker in the NDV F protein-mediated fusion process, 6 single amino acid mutants were obtained by site-directed mutagenesis of the HRB linker. The expression of the mutants and their abilities to mediate fusion were analysed, and the key amino acids in the HRB linker were identified as L436, E439, I450, and S453, as they can modulate the fusion ability or expression of the active form to a certain extent. The data shed light on the crucial role of the F protein HRB linker in the acquisition of a normal fusogenic phenotype.

Keywords: bacterial core genes;phylogenetic analysis;phylogenomics;phylogeny

Figure & Data

References

Citations

Citations to this article as recorded by

Evaluation of Newcastle disease virus LaSota strain attenuated by codon pair deoptimization of the HN and F genes for in ovo vaccination
Fatma Eldemery, Changbo Ou, Taejoong Kim, Stephen Spatz, John Dunn, Robert Silva, Qingzhong Yu
Veterinary Microbiology.2023; 277: 109625. CrossRef
Prevalence and Molecular Characterization of Bovine Parainfluenza Virus Type 3 in Cattle Herds in China
Yunxin Ren, Cheng Tang, Hua Yue
Animals.2023; 13(5): 793. CrossRef
Direct interaction of the molecular chaperone GRP78/BiP with the Newcastle disease virus hemagglutinin-neuraminidase protein plays a vital role in viral attachment to and infection of culture cells
Chenxin Han, Ziwei Xie, Yadi Lv, Dingxiang Liu, Ruiai Chen
Frontiers in Immunology.2023;[Epub] CrossRef
Adaptor complex-mediated trafficking of Newcastle disease virus fusion protein is regulated by the YLMY motif of its cytoplasmic tail
Yawen Bu, Qingyuan Teng, Delan Feng, Rong Liang, Haoran Wang, Xuehui Zhang, Xiao Li, Wenfeng Jia, Jia Xue, Ye Zhao, Guozhong Zhang
Virulence.2022; 13(1): 1849. CrossRef
YLMY Tyrosine Residue within the Cytoplasmic Tail of Newcastle Disease Virus Fusion Protein Regulates Its Surface Expression to Modulate Viral Budding and Pathogenicity
Yawen Bu, Qingyuan Teng, Delan Feng, Lu Sun, Jia Xue, Guozhong Zhang, Peter Pelka
Microbiology Spectrum.2021;[Epub] CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

The effect of the HRB linker of Newcastle disease virus fusion protein on the fusogenic activity

J. Microbiol. 2021;59(5):513-521. Published online March 29, 2021

DOI: https://doi.org/10.1007/s12275-021-0539-4

Related articles