Raman spectroscopy reveals alteration of spore compositions under different nutritional conditions in Lysinibacillus boronitolerans YS11

Youngung Ryu; Minyoung Hong; Soo Bin Kim; Tae Kwon Lee; Woojun Park

doi:10.1007/s12275-021-0679-6

Articles

Page Path: HOME > J. Microbiol > Volume 59(5); 2021 > Article

Journal Article
Raman spectroscopy reveals alteration of spore compositions under different nutritional conditions in Lysinibacillus boronitolerans YS11: Youngung Ryu ¹, Minyoung Hong ¹, Soo Bin Kim ², Tae Kwon Lee ², Woojun Park ¹; Journal of Microbiology 2021;59(5):491-499.
DOI: https://doi.org/10.1007/s12275-021-0679-6
Published online: March 29, 2021

¹Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Republic of Korea, ²Department of Environmental Engineering, Yonsei University, Wonju 26493, Republic of Korea¹Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Republic of Korea, ²Department of Environmental Engineering, Yonsei University, Wonju 26493, Republic of Korea

Corresponding author: Woojun Park , Tel: +82-2-3290-3067;,

Received: 22 December 2020 • Revised: 28 January 2021 • Accepted: 8 February 2021

prev next

74 Views
0 Download
7 Web of Science
7 Crossref
6 Scopus

Full Article

Abstract

Little is known about final spores components when bacteria undergo sporulation under different nutrient conditions. Different degrees of resistance and germination rates were observed in the three types of spores of Lysinibacillus boronitolerans YS11 (SD, Spores formed in Difco sporulation mediumTM; SC and SF, Spores formed in an agricultural byproduct medium with 10 mM CaCl2 and with 10 mM FeSO4, respectively). Stronger UV resistance was recorded for SF with 1.8–2.3-fold greater survival than SC and SD under UV treatment. The three spore types showed similar heat resistances at 80°C, but survival rates of SC and SD were much higher (~1,000 times) than those of SF at 90°C. However, germination capacity of SF was 20% higher than those of SD and SC on Luria-Bertani agar plates for 24 h. SF germinated more rapidly in a liquid medium with high NaCl concentrations than SC and SD, but became slower under alkaline conditions. Raman spectroscopy was used to analyze the heterogeneities in the three types of vegetative cells and their spores under different nutritional conditions. Exponentially grown-each vegetative cells had different overall Raman peak values. Raman peaks of SC, SD, and SF also showed differences in adenine and amide III compositions and nucleic acid contents. Our data along with Raman spectroscopy provided the evidence that spores formed under under different growth conditions possess very different cellular components, which affected their survival and germination rates.

Keywords: Escherichia coli BL21 (DE3);Phage contamination;Phage-resistant strains;Tn5

Figure & Data

References

Citations

Citations to this article as recorded by

Characterization of the Bacillus cereus spore killed by plasma-activated water (PAW)
Xiao Hu, Pengfei Ge, Xiaomeng Wang, Xinyu Liao, Jinsong Feng, Ruiling Lv, Tian Ding
Food Research International.2024; 196: 115058. CrossRef
Alleviation of H2O2 toxicity by extracellular catalases in the phycosphere of Microcystis aeruginosa
Yerim Park, Wonjae Kim, Yeji Cha, Minkyung Kim, Woojun Park
Harmful Algae.2024; 137: 102680. CrossRef
Effects of sporulation conditions on the growth, germination, and resistance of Clostridium perfringens spores
Dong Liang, Xiaoshuang Cui, Miaoyun Li, Yaodi Zhu, Lijun Zhao, Shijie Liu, Gaiming Zhao, Na Wang, Yangyang Ma, Lina Xu
International Journal of Food Microbiology.2023; 396: 110200. CrossRef
Lysinibacilli: A Biological Factories Intended for Bio-Insecticidal, Bio-Control, and Bioremediation Activities
Qazi Mohammad Sajid Jamal, Varish Ahmad
Journal of Fungi.2022; 8(12): 1288. CrossRef
Discrimination of Stressed and Non-Stressed Food-Related Bacteria Using Raman-Microspectroscopy
Daniel Klein, René Breuch, Jessica Reinmüller, Carsten Engelhard, Peter Kaul
Foods.2022; 11(10): 1506. CrossRef
Detection of low numbers of bacterial cells in a pharmaceutical drug product using Raman spectroscopy and PLS-DA multivariate analysis
R. A. Grosso, A. R. Walther, E. Brunbech, A. Sørensen, B. Schebye, K. E. Olsen, H. Qu, M. A. B. Hedegaard, E. C. Arnspang
The Analyst.2022; 147(15): 3593. CrossRef
Linkage between bacterial community-mediated hydrogen peroxide detoxification and the growth of Microcystis aeruginosa
Minkyung Kim, Wonjae Kim, Yunho Lee, Woojun Park
Water Research.2021; 207: 117784. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Raman spectroscopy reveals alteration of spore compositions under different nutritional conditions in Lysinibacillus boronitolerans YS11

J. Microbiol. 2021;59(5):491-499. Published online March 29, 2021

DOI: https://doi.org/10.1007/s12275-021-0679-6

Related articles