Crystal structure of the nuclease and capping domain of SbcD from Staphylococcus aureus

Jinwook Lee; Inseong Jo; Jinsook Ahn; Seokho Hong; Soyeon Jeong; Aeran Kwon; Nam-Chul Ha

doi:10.1007/s12275-021-1012-0

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Crystal structure of the nuclease and capping domain of SbcD from Staphylococcus aureus: Jinwook Lee ¹, Inseong Jo ¹, Jinsook Ahn ¹, Seokho Hong ¹, Soyeon Jeong ¹, Aeran Kwon ², Nam-Chul Ha ¹; Journal of Microbiology 2021;59(6):584-589.
DOI: https://doi.org/10.1007/s12275-021-1012-0
Published online: April 20, 2021

¹Research Institute of Agriculture and Life Sciences, Center for Food and Bioconvergence, Department of Agricultural Biotechnology, CALS, Seoul National University, Seoul 08826, Republic of Korea, ²Department of Beauty Care, College of Medical Science, Daegu Haany University, Gyeongsan 38610, Republic of Korea¹Research Institute of Agriculture and Life Sciences, Center for Food and Bioconvergence, Department of Agricultural Biotechnology, CALS, Seoul National University, Seoul 08826, Republic of Korea, ²Department of Beauty Care, College of Medical Science, Daegu Haany University, Gyeongsan 38610, Republic of Korea

Corresponding author: Nam-Chul Ha ,

Received: 8 January 2021 • Revised: 16 February 2021 • Accepted: 19 February 2021

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Abstract

The SbcCD complex is an essential component of the DNA double-strand break (DSB) repair system in bacteria. The bacterial SbcCD complex recognizes and cleaves the DNA ends in DSBs by ATP-dependent endo- and exonuclease activities as an early step of the DNA repair process. SbcD consists of nuclease, capping, and helix-loop-helix domains. Here, we present the crystal structure of a SbcD fragment from Staphylococcus aureus, which contained nuclease and capping domains, at a resolution of 2.9 Å. This structure shows a dimeric assembly similar to that of the corresponding domains of SbcD from Escherichia coli. The S. aureus SbcD fragment exhibited endonuclease activities on supercoiled DNA and exonuclease activity on linear and nicked DNA. This study contributes to the understanding of the molecular basis for how bacteria can resist sterilizing treatment, causing DNA damage.

Keywords: Epidemiology;Norovirus;Outbreak;Shenyang;Surveillance

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Staphylococcus aureus SOS response: Activation, impact, and drug targets
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Crystal structure of the nuclease and capping domain of SbcD from Staphylococcus aureus

J. Microbiol. 2021;59(6):584-589. Published online April 20, 2021

DOI: https://doi.org/10.1007/s12275-021-1012-0

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