Direct current exerts electricidal and bioelectric effects on Porphyromonas gingivalis biofilms partially via promoting oxidative stress and antibiotic transport

Peihui Zou; Peng Li; Jia Liu; Pei Cao; Qingxian Luan

doi:10.1007/s12275-022-1238-5

Articles

Page Path: HOME > J. Microbiol > Volume 60(1); 2022 > Article

Journal Article
Direct current exerts electricidal and bioelectric effects on Porphyromonas gingivalis biofilms partially via promoting oxidative stress and antibiotic transport: Peihui Zou ^1,2, Peng Li ^1,2, Jia Liu ^1,2, Pei Cao ^1,2, Qingxian Luan ^1,2; Journal of Microbiology 2022;60(1):70-78.
DOI: https://doi.org/10.1007/s12275-022-1238-5
Published online: November 26, 2021

¹Department of Periodontology, Peking University School and Hospital of Stomatology, National Center of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing 100081, P. R. China, ²Beijing Key Laboratory of Digital Stomatology, Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health, NMPA Key Laboratory for Dental Materials, Beijing 100081, P. R. China¹Department of Periodontology, Peking University School and Hospital of Stomatology, National Center of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing 100081, P. R. China, ²Beijing Key Laboratory of Digital Stomatology, Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health, NMPA Key Laboratory for Dental Materials, Beijing 100081, P. R. China

Received: 29 April 2021 • Revised: 5 October 2021 • Accepted: 12 October 2021

prev next

78 Views
0 Download
7 Web of Science
7 Crossref
7 Scopus

Full Article

Abstract

Low electric current can inhibit certain microbial biofilms and enhance the efficacy of antimicrobials against them. This study investigated the electricidal and bioelectric effects of direct current (DC) against Porphyromonas gingivalis biofilms as well as the underlying mechanisms. Here, we firstly showed that DC significantly suppressed biofilm formation of P. gingivalis in time- and intensity-dependent manners, and markedly inhibited preformed P. gingivalis biofilms. Moreover, DC enhanced the killing efficacy of metronidazole (MTZ) and amoxicillin with clavulanate potassium (AMC) against the biofilms. Notably, DC-treated biofilms displayed upregulated intracellular ROS and expression of ROS related genes (sod, feoB, and oxyR) as well as porin gene. Interestingly, DC-induced killing of biofilms was partially reversed by ROS scavenger N-dimethylthiourea (DMTU), and the synergistic effect of DC with MTZ/AMC was weakened by small interfering RNA of porin gene (si-Porin). In conclusion, DC can exert electricidal and bioelectric effects against P. gingivalis biofilms partially via promotion of oxidative stress and antibiotic transport, which offers a promising approach for effective management of periodontitis.

Keywords: Antifungal agents;Candida;Cryptococcus;Culture supernatant;Serratia marcescens

Figure & Data

References

Citations

Citations to this article as recorded by

Antifungal Activity, Synergism with Fluconazole or Amphotericin B and Potential Mechanism of Direct Current against Candida albicans Biofilms and Persisters
Peihui Zou, Jia Liu, Peng Li, Qingxian Luan
Antibiotics.2024; 13(6): 521. CrossRef
Smart dental materials for antimicrobial applications
Carolina Montoya, Lina Roldan, Michelle Yu, Sara Valliani, Christina Ta, Maobin Yang, Santiago Orrego
Bioactive Materials.2023; 24: 1. CrossRef
Molecular Study of Porphyromonas Gingivalis Strains With fimA Genotypes in Periodontitis Patients
Zhraa F. Faruq, Sami Khalaf Jabar
Iranian Journal of Medical Microbiology.2023; 17(6): 663. CrossRef
Drug delivery approaches for enhanced antibiofilm therapy
Tao Wang, Erik Jan Cornel, Chang Li, Jianzhong Du
Journal of Controlled Release.2023; 353: 350. CrossRef
Weak direct current exerts synergistic effect with antibiotics and reduces the antibiotic resistance: An in vitro subgingival plaque biofilm model
Peihui Zou, Yanfeng Wang, Pei Cao, Peng Li, Jia Liu, Qingxian Luan
Journal of Periodontal Research.2023; 58(1): 143. CrossRef
Bioelectric device for effective biofilm inflammation management of dental implants
Jihyun Lee, Young Wook Kim
Scientific Reports.2023;[Epub] CrossRef
A Highly Efficacious Electrical Biofilm Treatment System for Combating Chronic Wound Bacterial Infections
Fan Zhao, Yajuan Su, Junying Wang, Svetlana Romanova, Dominick J. DiMaio, Jingwei Xie, Siwei Zhao
Advanced Materials.2023;[Epub] CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Direct current exerts electricidal and bioelectric effects on Porphyromonas gingivalis biofilms partially via promoting oxidative stress and antibiotic transport

J. Microbiol. 2022;60(1):70-78. Published online November 26, 2021

DOI: https://doi.org/10.1007/s12275-022-1238-5

Related articles