Two Forms of Vibrio vulnificus Metalloprotease VvpE are Secreted via the Type II General Secretion System

Jong Park; So-Yeon Ryu; Choon-Mee Kim; Sung-Heui Shin

doi:10.1007/s12275-008-0058-6

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Two Forms of Vibrio vulnificus Metalloprotease VvpE are Secreted via the Type II General Secretion System: Jong Park ^1,2, So-Yeon Ryu ^1,2, Choon-Mee Kim ¹, Sung-Heui Shin ^1,3; Journal of Microbiology 2008;46(3):338-343.
DOI: https://doi.org/10.1007/s12275-008-0058-6
Published online: July 5, 2008

¹Research Center for Resistant Cells, Chosun University Medical School, Gwangju 501-759, Republic of Korea, ²Department of Preventive Medicine, Chosun University Medical School, Gwangju 501-759, Republic of Korea, ³Department of Microbiology, Chosun University Medical School, Gwangju 501-759, Republic of Korea

Corresponding author: Sung-Heui Shin , Tel: 82-62-230-6352,

Received: 29 February 2008 • Accepted: 22 April 2008

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Abstract

Vibrio vulnificus has been known to secrete one form of metalloprotease VvpE (45 kDa) that is cleaved to 34 kDa-VvpE and 11 kDa-C-terminal propeptide via extracellular autoproteolysis. However, we found that extracellular secretion of both the 34 and 45 kDa forms of VvpE began in the early growth phase; moreover, 34 kDa-VvpE existed as the major form in V. vulnificus cell lysates and culture supernatants. In addition, extracellular secretion of both 34 and 45 kDa-VvpE was blocked by mutation of the pilD gene, which encodes for the type IV leader peptidase/N-methyltransferase of the type II general secretion system, and the blocked VvpE secretion was recovered by in trans-complementation of the wild-type pilD gene. These results indicate that 34 kDa-VvpE is the major form secreted along with 45 kDa-VvpE from the early growth phase via the PilD-mediated type II general secretion system.

Keywords: Vibrio vulnificus;metalloprotease;general secretion system

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Two Forms of Vibrio vulnificus Metalloprotease VvpE are Secreted via the Type II General Secretion System

J. Microbiol. 2008;46(3):338-343. Published online July 5, 2008

DOI: https://doi.org/10.1007/s12275-008-0058-6

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