Cloning, Expression, and Characterization of Xylose Reductase with Higher Activity from Candida tropicalis

Feiwei Zhang; Dairong Qiao; Hui Xu; Chong Liao; Shilin Li; Yi Cao

doi:10.1007/s12275-008-0225-9

Articles

Page Path: HOME > J. Microbiol > Volume 47(3); 2009 > Article

Research Support, Non-U.S. Gov't
Cloning, Expression, and Characterization of Xylose Reductase with Higher Activity from Candida tropicalis: Feiwei Zhang ¹, Dairong Qiao ^1,2, Hui Xu ¹, Chong Liao ¹, Shilin Li ¹, Yi Cao ^1,2; Journal of Microbiology 2009;47(3):351-357.
DOI: https://doi.org/10.1007/s12275-008-0225-9
Published online: June 26, 2009

¹College of Life Sciences, Sichuan University, Chengdu 610064, P. R. China, ²Sichuan Public Experimental Platform of Bioinformatics and Metabolic Engineering, Chengdu 610064, P. R. China¹College of Life Sciences, Sichuan University, Chengdu 610064, P. R. China, ²Sichuan Public Experimental Platform of Bioinformatics and Metabolic Engineering, Chengdu 610064, P. R. China

Corresponding author: Yi Cao , Tel: 86-028-8541-2842,

Received: 16 September 2008 • Accepted: 23 March 2009

prev next

43 Views
0 Download
17 Crossref
25 Scopus

Full Article

Abstract

Xylose reductase (XR) is a key enzyme in xylose metabolism because it catalyzes the reduction of xylose to xylitol. In order to study the characteristics of XR from Candida tropicalis SCTCC 300249, its XR gene(xyl1) was cloned and expressed in Escherichia coli BL21 (DE3). The fusion protein was purified effectively by Ni2+-chelating chromatography, and the kinetics of the recombinant XR was investigated. The Km values of the C. tropicalis XR for NADPH and NADH were 45.5 uM and 161.9 uM, respectively, which demonstrated that this XR had dual coenzyme specificity. Moreover, this XR showed the highest catalytic efficiency (kcat=1.44x04 min-1) for xylose among the characterized aldose reductases. Batch fermentation was performed with Saccharomyces serivisiae W303-1A:pYES2XR, and resulted in 7.63 g/L cell mass, 93.67 g/L xylitol, and 2.34 g/Lh xylitol productivity. This XR coupled with its dual coenzyme specificity, high activity, and catalytic efficiency proved its utility in in vitro xylitol production.

Keywords: Candida tropicalis;xylose reductase;coenzyme specificity;catalytic efficiency

Figure & Data

References

Citations

Citations to this article as recorded by

Efficient production of ginsenoside Rh2 from xylose by remodeling metabolism in Saccharomyces cerevisiae
Wanze Zhang, Jiale Zhang, Xiaomeng Zhao, Zhanwei Zhang, Shifan He, Xueke Bian, Haibin Wang, Chuanbo Zhang, Wenyu Lu
Chemical Engineering Journal.2024; 494: 153120. CrossRef
Recent insights, applications and prospects of xylose reductase: a futuristic enzyme for xylitol production
Yogita Lugani, Munish Puri, Balwinder Singh Sooch
European Food Research and Technology.2021; 247(4): 921. CrossRef
Characterization of d-xylose reductase, XyrB, from Aspergillus niger
Agata Terebieniec, Tania Chroumpi, Adiphol Dilokpimol, Maria Victoria Aguilar-Pontes, Miia R. Mäkelä, Ronald P. de Vries
Biotechnology Reports.2021; 30: e00610. CrossRef
Combining Xylose Reductase from Spathaspora arborariae with Xylitol Dehydrogenase from Spathaspora passalidarum to Promote Xylose Consumption and Fermentation into Xylitol by Saccharomyces cerevisiae
Adriane Mouro, Angela A. dos Santos, Denis D. Agnolo, Gabriela F. Gubert, Elba P. S. Bon, Carlos A. Rosa, César Fonseca, Boris U. Stambuk
Fermentation.2020; 6(3): 72. CrossRef
Kinetics and Predicted Structure of a Novel Xylose Reductase from Chaetomium thermophilum
Julian Quehenberger, Tom Reichenbach, Niklas Baumann, Lukas Rettenbacher, Christina Divne, Oliver Spadiut
International Journal of Molecular Sciences.2019; 20(1): 185. CrossRef
Biosynthetic strategies to produce xylitol: an economical venture
Yirong Xu, Ping Chi, Muhammad Bilal, Hairong Cheng
Applied Microbiology and Biotechnology.2019; 103(13): 5143. CrossRef
A halotolerant aldose reductase from Debaryomyces nepalensis: gene isolation, overexpression and biochemical characterization
Bhaskar Paidimuddala, Gopala Krishna Aradhyam, Sathyanarayana N. Gummadi
RSC Advances.2017; 7(33): 20384. CrossRef
Inhibition of Debaryomyces nepalensis xylose reductase by lignocellulose derived by-products
Bhaskar Paidimuddala, Ashish Rathod, Sathyanarayana N. Gummadi
Biochemical Engineering Journal.2017; 121: 73. CrossRef
Bioprospecting and evolving alternative xylose and arabinose pathway enzymes for use in Saccharomyces cerevisiae
Sun-Mi Lee, Taylor Jellison, Hal S. Alper
Applied Microbiology and Biotechnology.2016; 100(5): 2487. CrossRef
The yeast Scheffersomyces amazonensis is an efficient xylitol producer
Raquel M. Cadete, Monaliza A. Melo-Cheab, Adriana L. Viana, Evelyn S. Oliveira, César Fonseca, Carlos A. Rosa
World Journal of Microbiology and Biotechnology.2016;[Epub] CrossRef
Identification and characterization of d-arabinose reductase and d-arabinose transporters from Pichia stipitis
Seiya Watanabe, Yuki Utsumi, Shigeki Sawayama, Yasuo Watanabe
Bioscience, Biotechnology, and Biochemistry.2016; 80(11): 2151. CrossRef
Sequence analysis of KmXYL1 genes and verification of thermotolerant enzymatic activities of xylose reductase from four Kluyveromyces marxianus strains
Jae-Bum Park, Jin-Seong Kim, Seung-Won Jang, Deok-Ho Kweon, Eock Kee Hong, Won Cheol Shin, Suk-Jin Ha
Biotechnology and Bioprocess Engineering.2016; 21(5): 581. CrossRef
Cloning, expression, and characterization of a novel xylose reductase from Rhizopus oryzae
Min Zhang, Shao‐tong Jiang, Zhi Zheng, Xing‐jiang Li, Shui‐zhong Luo, Xue‐feng Wu
Journal of Basic Microbiology.2015; 55(7): 907. CrossRef
Metabolic engineering strategies for improving xylitol production from hemicellulosic sugars
Buli Su, Mianbin Wu, Jianping Lin, Lirong Yang
Biotechnology Letters.2013; 35(11): 1781. CrossRef
Identification of a xylose reductase gene in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777
Biao Zhang, Ling Zhang, Dongmei Wang, Xiaolian Gao, Jiong Hong
Journal of Industrial Microbiology & Biotechnology.2011; 38(12): 2001. CrossRef
Purification and biochemical characterization of a moderately halotolerant NADPH dependent xylose reductase from Debaryomyces nepalensis NCYC 3413
Sawan Kumar, Sathyanarayana N. Gummadi
Bioresource Technology.2011; 102(20): 9710. CrossRef
Cosubstrate effect on xylose reductase and xylitol dehydrogenase activity levels, and its consequence on xylitol production by Candida tropicalis
Elena Tamburini, Ercolina Bianchini, Alessandro Bruni, Giuseppe Forlani
Enzyme and Microbial Technology.2010; 46(5): 352. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Cloning, Expression, and Characterization of Xylose Reductase with Higher Activity from Candida tropicalis

J. Microbiol. 2009;47(3):351-357. Published online June 26, 2009

DOI: https://doi.org/10.1007/s12275-008-0225-9

Related articles