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- Furan-based Chalcone Annihilates the Multi-Drug-Resistant Pseudomonas aeruginosa and Protects Zebra Fish Against its Infection
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Santosh Pushpa Ramya Ranjan Nayak , Catharine Basty , Seenivasan Boopathi , Loganathan Sumathi Dhivya , Khaloud Mohammed Alarjani , Mohamed Ragab Abdel Gawwad , Raghda Hager , Muthu Kumaradoss Kathiravan , Jesu Arockiaraj
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J. Microbiol. 2024;62(2):75-89. Published online February 21, 2024
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DOI: https://doi.org/10.1007/s12275-024-00103-6
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Abstract
- The emergence of carbapenem-resistant Pseudomonas aeruginosa, a multi-drug-resistant bacteria, is becoming a serious
public health concern. This bacterium infects immunocompromised patients and has a high fatality rate. Both naturally and
synthetically produced chalcones are known to have a wide array of biological activities. The antibacterial properties of
synthetically produced chalcone were studied against P. aeruginosa. In vitro, study of the compound (chalcone derivative
named DKO1), also known as (2E)-1-(5-methylfuran-2-yl)-3-(4-nitrophenyl) prop-2-en-1-one, had substantial antibacterial
and biofilm disruptive action. DKO1 effectively shielded against P. aeruginosa-induced inflammation, oxidative stress, lipid
peroxidation, and apoptosis in zebrafish larvae. In adult zebrafish, the treatment enhanced the chances of survivability and
reduced the sickness-like behaviors. Gene expression, biochemical analysis, and histopathology studies found that proinflammatory
cytokines (TNF-α, IL-1β, IL-6, iNOS) were down regulated; antioxidant enzymes such as superoxide dismutase
(SOD) and catalase (CAT) levels increased, and histoarchitecture was restored in zebrafish. The data indicate that DKO1 is
an effective antibacterial agent against P. aeruginosa demonstrated both in vitro and in vivo.
- Ultrasonic Treatment Enhanced Astaxanthin Production of Haematococcus pluvialis
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Yun Hwan Park , Jaewon Park , Jeong Sik Choi , Hyun Soo Kim , Jong Soon Choi , Yoon-E Choi
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J. Microbiol. 2023;61(6):633-639. Published online June 13, 2023
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DOI: https://doi.org/10.1007/s12275-023-00053-5
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Abstract
- In this study, effects of ultrasonic treatment on Haematococcus pluvialis (H. pluvialis) were investigated. It has been confirmed
that the ultrasonic stimulation acted as stress resources in the red cyst stage H. pluvialis cells containing astaxanthin,
result
ing in additional astaxanthin production. With the increase in production of astaxanthin, the average diameter of H.
pluvialis cells increased accordingly. In addition, to determine how ultrasonic stimulation had an effect on the further biosynthesis
of astaxanthin, genes related to astaxanthin synthesis and cellular ROS level were measured. As a result, it was
confirmed that astaxanthin biosynthesis related genes and cellular ROS levels were increased, and thus ultrasonic stimulation
acts as an oxidative stimulus. These results support the notion on the effect of the ultrasonic treatment, and we believe
our novel approach based on the ultrasonic treatment would help to enhance the astaxanthin production from H. pluvialis.
Review
- The crosstalk between bacteria and host autophagy: host defense or bacteria offense
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Lin Zheng , Fang Wei , Guolin Li
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J. Microbiol. 2022;60(5):451-460. Published online April 29, 2022
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DOI: https://doi.org/10.1007/s12275-022-2009-z
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6
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Abstract
- Xenophagy is a specific selective autophagy for the elimination
of intracellular bacteria. Current evidence suggests that the
processes for host autophagy system to recognize and eliminate
invading bacteria are complex, and vary according to
different pathogens. Although both ubiquitin-dependent and
ubiquitin-independent autophagy exist in host to defense invading
bacteria, successful pathogens have evolved diverse
strategies to escape from or paralyze host autophagy system.
In this review, we discuss the mechanisms of host autophagy
system to recognize and eliminate intracellular pathogens and
the mechanisms of different pathogens to escape from or paralyze
host autophagy system, with a particular focus on the
most extensively studied bacteria.
Journal Article
- [PROTOCOL]A Signature-Tagged Mutagenesis (STM)-based murine-infectivity assay for Cryptococcus neoformans
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Kwang-Woo Jung , Kyung-Tae Lee , Yong-Sun Bahn
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J. Microbiol. 2020;58(10):823-831. Published online September 29, 2020
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DOI: https://doi.org/10.1007/s12275-020-0341-8
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Abstract
- Signature-tagged mutagenesis (STM) is a high-throughput
genetic technique that can be used to investigate the function
of genes by constructing a large number of mutant strains
with unique DNA identification tags, pooling them, and screening
them for a particular phenotypic trait. STM was first designed
for the identification of genes that contribute to the
virulence or infectivity of a pathogen in its host. Recently, this
method
has also been applied for the identification of mutants
with specific phenotypes, such as antifungal drug resistance
and proliferation. In the present study, we describe an STM
method
for the identification of genes contributing to the infectivity
of Cryptococcus neoformans using a mutant library,
in which each strain was tagged with a unique DNA sequence.
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