The quorum sensing regulator OpaR is a repressor of polar flagellum genes in Vibrio parahaemolyticus

Renfei Lu; Junfang Sun; Yue Qiu; Miaomiao Zhang; Xingfan Xue; Xue Li; Wenhui Yang; Dongsheng Zhou; Lingfei Hu; Yiquan Zhang

doi:10.1007/s12275-021-0629-3

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The quorum sensing regulator OpaR is a repressor of polar flagellum genes in Vibrio parahaemolyticus: Renfei Lu ¹, Junfang Sun ¹, Yue Qiu ³, Miaomiao Zhang ³, Xingfan Xue ³, Xue Li ¹, Wenhui Yang ⁴, Dongsheng Zhou ⁴, Lingfei Hu ⁴, Yiquan Zhang ^2,3; Journal of Microbiology 2021;59(7):651-657.
DOI: https://doi.org/10.1007/s12275-021-0629-3
Published online: June 1, 2021

¹Department of Clinical Laboratory, Nantong Third Hospital Affiliated to Nantong University, Nantong 212006, P. R. China, ²Wuxi School of Medicine, Jiangnan University, Wuxi 214122, P. R. China, ³School of Medicine, Jiangsu University, Zhenjiang 212013, P. R. China, ⁴State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China¹Department of Clinical Laboratory, Nantong Third Hospital Affiliated to Nantong University, Nantong 212006, P. R. China, ²Wuxi School of Medicine, Jiangnan University, Wuxi 214122, P. R. China, ³School of Medicine, Jiangsu University, Zhenjiang 212013, P. R. China, ⁴State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China

Corresponding author: Lingfei Hu ,
Yiquan Zhang ,

Received: 1 December 2020 • Revised: 29 March 2021 • Accepted: 22 April 2021

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Abstract

Vibrio parahaemolyticus possesses two types of flagella: a single polar flagellum (Pof) for swimming and the peritrichous lateral flagella (Laf) for swarming. Expression of Laf genes has previously been reported to be regulated by the quorum sensing (QS) regulators AphA and OpaR. In the present study, we showed that OpaR, the QS regulator at high cell density (HCD), acted as a negative regulator of swimming motility and the transcription of Pof genes in V. parahaemolyticus. OpaR bound to the promoter-proximal DNA regions of flgAMN, flgMN, and flgBCDEFGHIJ within the Pof gene loci to repress their transcription, whereas it negatively regulates the transcription of flgKL-flaC in an indirect manner. Thus, this work investigated how QS regulated the swimming motility via direct action of its master regulator OpaR on the transcription of Pof genes in V. parahaemolyticus.

Keywords: Antarctic lichen diversity;Ecological traits;Lichen;Lichen taxonomy

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The quorum sensing regulator OpaR is a repressor of polar flagellum genes in Vibrio parahaemolyticus

J. Microbiol. 2021;59(7):651-657. Published online June 1, 2021

DOI: https://doi.org/10.1007/s12275-021-0629-3

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