Journal of Microbiology

Journal Articles

Comparative Secretory Efficiency of Two Chitosanase Signal Peptides from Bacillus subtilis in Escherichia coli: Tae-Yang Eom, Yehui Gang, Youngdeuk Lee, Yoon-Hyeok Kang, Eunyoung Jo, Svini Dileepa Marasinghe, Heung Sik Park, Gun-Hoo Park, Chulhong Oh; J. Microbiol. 2024;62(12):1155-1164. Published online November 25, 2024; DOI: https://doi.org/10.1007/s12275-024-00186-1

20 View
0 Download

Abstract: The production of recombinant proteins in Escherichia coli is often challenged by cytoplasmic expression due to proteolytic degradation and inclusion body formation. Extracellular expression can overcome these problems by simplifying downstream processing and improving protein yields. This study aims to compare the efficiency of two Bacillus subtilis chitosanase signal peptides in mediating extracellular secretion in E. coli. We identified a naturally occurring mutant signal peptide (mCsn2-SP) from B. subtilis CH2 chitosanase (CH2CSN), which is characterized by a deletion of six amino acids in the N-region relative to the signal peptide (Csn1-SP) from B. subtilis CH1 chitosanase (CH1CSN). The CH1CSN and CH2CSN genes were cloned into the pET-11a vector and protein secretion was evaluated in E. coli BL21(DE3) host cells. Expression was induced with 0.1 mM and 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) at 30 °C for one and three days. CH2CSN showed higher secretion levels compared to CH1CSN under all experimental conditions, especially with 0.1 mM IPTG induction for 3 days, which resulted in a 2.37-fold increase in secretion. Furthermore, it was demonstrated that mCsn2-SP is capable of secreting human Cu,Zn-superoxide dismutase (hSOD) in E. coli BL21(DE3) and successfully translocating it to the periplasmic region. This study represents the inaugural investigation into the utilisation of a naturally modified signal peptide, thereby corroborating the assertion that signal peptide deletion variants can influence protein secretion efficiency. Furthermore, the findings substantiate the proposition that such variants can serve as a viable alternative for the secretion of heterologous proteins in E. coli.

Relaxed Cleavage Specificity of Hyperactive Variants of Escherichia coli RNase E on RNA I: Dayeong Bae , Hana Hyeon , Eunkyoung Shin , Ji&# , Kangseok Lee; J. Microbiol. 2023;61(2):211-220. Published online February 22, 2023; DOI: https://doi.org/10.1007/s12275-023-00013-z

24 View
0 Download
1 Web of Science
1 Crossref

RNase E is an essential enzyme in Escherichia coli. The cleavage site of this single-stranded specific endoribonuclease is well-characterized in many RNA substrates. Here, we report that the upregulation of RNase E cleavage activity by a mutation that affects either RNA binding (Q36R) or enzyme multimerization (E429G) was accompanied by relaxed cleavage specificity. Both mutations led to enhanced RNase E cleavage in RNA I, an antisense RNA of ColE1-type plasmid replication, at a major site and other cryptic sites. Expression of a truncated RNA I with a major RNase E cleavage site deletion at the 5′-end (RNA I- 5) resulted in an approximately twofold increase in the steady-state levels of RNA I- 5 and the copy number of ColE1-type plasmid in E. coli cells expressing wild-type or variant RNase E compared to those expressing RNA I. These
results
indicate that RNA I- 5 does not efficiently function as an antisense RNA despite having a triphosphate group at the 5′-end, which protects the RNA from ribonuclease attack. Our study suggests that increased cleavage rates of RNase E lead to relaxed cleavage specificity on RNA I and the inability of the cleavage product of RNA I as an antisense regulator in vivo does not stem from its instability by having 5′-monophosphorylated end.

Citations

Citations to this article as recorded by

Engineering an Escherichia coli based in vivo mRNA manufacturing platform
Edward Curry, George Muir, Jixin Qu, Zoltán Kis, Martyn Hulley, Adam Brown
Biotechnology and Bioengineering.2024; 121(6): 1912. CrossRef

The novel antifungal agent AB-22 displays in vitro activity against hyphal growth and biofilm formation in Candida albicans and potency for treating systemic candidiasis: Kyung-Tae Lee , Dong-Gi Lee , Ji Won Choi , Jong-Hyun Park , Ki Duk Park , Jong-Seung Lee , Yong-Sun Bahn; J. Microbiol. 2022;60(4):438-443. Published online March 14, 2022; DOI: https://doi.org/10.1007/s12275-022-2016-0

22 View
0 Download
1 Web of Science
1 Scopus

Abstract: Systemic candidiasis, which is mainly caused by Candida albicans, is a serious acute fungal infection in the clinical setting. In a previous study, we reported that compound 22h (designated as AB-22 in this study), a vinyl sulfate compound, is a fast-acting fungicidal agent against a broad spectrum of fungal pathogens. In this study, we aimed to further analyze the in vitro and in vivo efficacy of AB-22 against filamentation, biofilm formation, and virulence of C. albicans. Under in vitro hyphal growth-inducing condition, AB-22 effectively inhibited germ tube formation and hyphal growth, which are required for the initiation of biofilm formation. Indeed, AB-22 significantly suppressed C. albicans biofilm formation in a dose-dependent manner. Moreover, AB-22 treatment inhibited the normal induction of ALS3, HWP1, and ECE1, which are all required for hyphal transition in C. albicans. Furthermore, AB-22 treatment increased the survival of mice systemically infected with C. albicans. In conclusion, in addition to its fungicidal activity, AB-22 inhibits filamentation and biofilm formation in C. albicans, which could collectively contribute to its potent in vivo efficacy against systemic candidiasis.

Novosphingobium sp. PP1Y as a novel source of outer membrane vesicles: Federica De Lise , Francesca Mensitieri , Giulia Rusciano , Fabrizio Dal Piaz , Giovanni Forte , Flaviana Di Lorenzo , Antonio Molinaro , Armando Zarrelli , Valeria Romanucci , Valeria Cafaro , Antonio Sasso , Amelia Filippelli , Alberto Di Donato , Viviana Izzo; J. Microbiol. 2019;57(6):498-508. Published online May 27, 2019; DOI: https://doi.org/10.1007/s12275-019-8483-2

21 View
0 Download
4 Web of Science
4 Crossref

Abstract

Outer membrane vesicles (OMVs) are nanostructures of 20– 200 nm diameter deriving from the surface of several Gramnegative bacteria. OMVs are emerging as shuttles involved in several mechanisms of communication and environmental adaptation. In this work, OMVs were isolated and characterized from Novosphingobium sp. PP1Y, a Gram-negative non-pathogenic microorganism lacking LPS on the outer membrane surface and whose genome was sequenced and annotated. Scanning electron microscopy performed on samples obtained from a culture in minimal medium highlighted the presence of PP1Y cells embedded in an extracellular matrix rich in vesicular structures. OMVs were collected from the exhausted growth medium during the mid-exponential phase, and purified by ultracentrifugation on a sucrose gradient. Atomic force microscopy, dynamic light scattering and nanoparticle tracking analysis showed that purified PP1Y OMVs had a spherical morphology with a diameter of ca. 150 nm and were homogenous in size and shape. Moreover, proteomic and fatty acid analysis of purified OMVs revealed a specific biochemical “fingerprint”, suggesting interesting details concerning their biogenesis and physiological role. Moreover, these extracellular nanostructures do not appear to be cytotoxic on HaCaT cell line, thus paving the way to their future use as novel drug delivery systems.

Citations

Citations to this article as recorded by

Proteomic analysis of meropenem-induced outer membrane vesicles released by carbapenem-resistant Klebsiella pneumoniae
Fangfang Fan, Guangzhang Chen, Siqian Deng, Li Wei, Mariola J. Ferraro
Microbiology Spectrum.2024;[Epub] CrossRef
LuxR402 of Novosphingobium sp. HR1a regulates the correct configuration of cell envelopes
Ana Segura, Lázaro Molina
Frontiers in Microbiology.2023;[Epub] CrossRef
Genomic and physiological characterization of Novosphingobium terrae sp. nov., an alphaproteobacterium isolated from Cerrado soil containing a mega-sized chromid
Aline Belmok, Felipe Marques de Almeida, Rodrigo Theodoro Rocha, Carla Simone Vizzotto, Marcos Rogério Tótola, Marcelo Henrique Soller Ramada, Ricardo Henrique Krüger, Cynthia Maria Kyaw, Georgios J. Pappas
Brazilian Journal of Microbiology.2023; 54(1): 239. CrossRef
Outer Membrane Vesicles Derived from Klebsiella pneumoniae Are a Driving Force for Horizontal Gene Transfer
Federica Dell’Annunziata, Carmela Dell’Aversana, Nunzianna Doti, Giuliana Donadio, Fabrizio Dal Piaz, Viviana Izzo, Anna De Filippis, Marilena Galdiero, Lucia Altucci, Giovanni Boccia, Massimiliano Galdiero, Veronica Folliero, Gianluigi Franci
International Journal of Molecular Sciences.2021; 22(16): 8732. CrossRef

ZntR positively regulates T6SS4 expression in Yersinia pseudotuberculosis: Tietao Wang , Keqi Chen , Fen Gao , Yiwen Kang , Muhammad Tausif Chaudhry , Zhuo Wang , Yao Wang , Xihui Shen; J. Microbiol. 2017;55(6):448-456. Published online March 10, 2017; DOI: https://doi.org/10.1007/s12275-017-6540-2

23 View
0 Download
19 Crossref

Abstract

The type VI secretion system (T6SS) is a widespread and versatile protein secretion system found in most Gram- negative bacteria. Studies of T6SS have mainly focused on its role in virulence toward host cells and inter-bacterial inter-actions, but studies have also shown that T6SS4 in Yersinia pseudotuberculosis participates in the acquisition of zinc ions to alleviate the accumulation of hydroxyl radicals induced by multiple stressors. Here, by comparing the gene expression patterns of wild-type and zntR mutant Y. pseudotubercu-losis cells using RNA-seq analysis, T6SS4 and 17 other bio-logical processes were found to be regulated by ZntR. T6SS4 was positively regulated by ZntR in Y. pseudotuberculosis, and further investigation demonstrated that ZntR regulates T6SS4 by directly binding to its promoter region. T6SS4 ex-pression is regulated by zinc via ZntR, which maintains in-tracellular zinc homeostasis and controls the concentration of reactive oxygen species to prevent bacterial death under oxidative stress. This study provides new insights into the regulation of T6SS4 by a zinc-dependent transcriptional regu-lator, and it provides a foundation for further investigation of the mechanism of zinc transport by T6SS.

Citations

Citations to this article as recorded by

Two-Component Signaling System RegAB Represses Pseudomonas syringae pv. actinidiae T3SS by Directly Binding to the promoter of hrpRS1
Mengsi Zhang, Mingming Yang, Xiaoxue Zhang, Shuying Li, Shuaiwu Wang, Alex Muremi Fulano, Yongting Meng, Xihui Shen, Li-li Huang, Yao Wang
Journal of Integrative Agriculture.2024;[Epub] CrossRef
Genome-wide phenotypic profiling of transcription factors and identification of novel targets to control the virulence of Vibrio vulnificus
Dayoung Sung, Garam Choi, Minji Ahn, Hokyung Byun, Tae Young Kim, Hojun Lee, Zee-Won Lee, Ji Yong Park, Young Hyun Jung, Ho Jae Han, Sang Ho Choi
Nucleic Acids Research.2024;[Epub] CrossRef
Pb2+ biosorption by Serratia marcescens CCMA 1010 and its relation with zntR gene expression and ZntA efflux pump regulation
Jorge Dias Carlier, Gustavo Magno dos Reis Ferreira, Rosane Freitas Schwan, Cristina Ferreira da Silva, Maria Clara Costa
Environmental Advances.2024; 15: 100479. CrossRef
OxyR-regulated T6SS functions in coordination with siderophore to resist oxidative stress
Changfu Li, Zhiyan Wei, Xinquan He, Haiyang He, Yuqi Liu, Yuxin Zuo, He Xiao, Yao Wang, Xihui Shen, Lingfang Zhu, Olaya Rendueles
Microbiology Spectrum.2024;[Epub] CrossRef
A σE-mediated temperature gauge orchestrates type VI secretion system, biofilm formation and cell invasion in pathogen Pseudomonas plecoglossicida
Yibei Zhang, Yuping Huang, Haoyuan Ding, Jiabao Ma, Xinyu Tong, Yuanxing Zhang, Zhen Tao, Qiyao Wang
Microbiological Research.2023; 266: 127220. CrossRef
Impact of lead (Pb2+) on the growth and biological activity of Serratia marcescens selected for wastewater treatment and identification of its zntR gene—a metal efflux regulator
Gustavo Magno dos Reis Ferreira, Josiane Ferreira Pires, Luciana Silva Ribeiro, Jorge Dias Carlier, Maria Clara Costa, Rosane Freitas Schwan, Cristina Ferreira Silva
World Journal of Microbiology and Biotechnology.2023;[Epub] CrossRef
MlrA, a MerR family regulator in Vibrio cholerae , senses the anaerobic signal in the small intestine of the host to promote bacterial intestinal colonization
Jialin Wu, Yutao Liu, Wendi Li, Fan Li, Ruiying Liu, Hao Sun, Jingliang Qin, Xiaohui Feng, Di Huang, Bin Liu
Gut Microbes.2022;[Epub] CrossRef
Nutritional immunity: the battle for nutrient metals at the host–pathogen interface
Caitlin C. Murdoch, Eric P. Skaar
Nature Reviews Microbiology.2022; 20(11): 657. CrossRef
The transcriptional regulator Zur regulates the expression of ZnuABC and T6SS4 in response to stresses in Yersinia pseudotuberculosis
Ran Cai, Fen Gao, Junfeng Pan, Xinwei Hao, Zonglan Yu, Yichen Qu, Jialin Li, Dandan Wang, Yao Wang, Xihui Shen, Xingyu Liu, Yantao Yang
Microbiological Research.2021; 249: 126787. CrossRef
T6SS Mediated Stress Responses for Bacterial Environmental Survival and Host Adaptation
Kai-Wei Yu, Peng Xue, Yang Fu, Liang Yang
International Journal of Molecular Sciences.2021; 22(2): 478. CrossRef
Yersiniabactin contributes to overcoming zinc restriction during Yersinia pestis infection of mammalian and insect hosts
Sarah L. Price, Viveka Vadyvaloo, Jennifer K. DeMarco, Amanda Brady, Phoenix A. Gray, Thomas E. Kehl-Fie, Sylvie Garneau-Tsodikova, Robert D. Perry, Matthew B. Lawrenz
Proceedings of the National Academy of Sciences.2021;[Epub] CrossRef
Roles of Type VI Secretion System in Transport of Metal Ions
Xiaobing Yang, Hai Liu, Yanxiong Zhang, Xihui Shen
Frontiers in Microbiology.2021;[Epub] CrossRef
Beyond dueling: roles of the type VI secretion system in microbiome modulation, pathogenesis and stress resistance
Jinshui Lin, Lei Xu, Jianshe Yang, Zhuo Wang, Xihui Shen
Stress Biology.2021;[Epub] CrossRef
Coordinated regulation of anthranilate metabolism and bacterial virulence by the GntR family regulator MpaR in Pseudomonas aeruginosa
Tietao Wang, Yihang Qi, Zhihan Wang, Jingru Zhao, Linxuan Ji, Jun Li, Zhao Cai, Liang Yang, Min Wu, Haihua Liang
Molecular Microbiology.2020; 114(5): 857. CrossRef
RovC - a novel type of hexameric transcriptional activator promoting type VI secretion gene expression
Vanessa Knittel, Pooja Sadana, Stephanie Seekircher, Anne-Sophie Stolle, Britta Körner, Marcel Volk, Cy M. Jeffries, Dmitri I. Svergun, Ann Kathrin Heroven, Andrea Scrima, Petra Dersch, Joan Mecsas
PLOS Pathogens.2020; 16(9): e1008552. CrossRef
The type VI secretion system protein AsaA in Acinetobacter baumannii is a periplasmic protein physically interacting with TssM and required for T6SS assembly
Lei Li, Yi-Nuo Wang, Hong-Bing Jia, Ping Wang, Jun-Fang Dong, Juan Deng, Feng-Min Lu, Qing-Hua Zou
Scientific Reports.2019;[Epub] CrossRef
Confirmed and Potential Roles of Bacterial T6SSs in the Intestinal Ecosystem
Can Chen, Xiaobing Yang, Xihui Shen
Frontiers in Microbiology.2019;[Epub] CrossRef
The stringent response factor, RelA, positively regulates T6SS4 expression through the RovM/RovA pathway in Yersinia pseudotuberculosis
Xiaobing Yang, Yunhong Song, Qingyun Dai, Hongyun Zhang, Li Song, Zhuo Wang, Junfeng Pan, Yao Wang
Microbiological Research.2019; 220: 32. CrossRef
Type VI Secretion Systems Present New Insights on Pathogenic Yersinia
Xiaobing Yang, Junfeng Pan, Yao Wang, Xihui Shen
Frontiers in Cellular and Infection Microbiology.2018;[Epub] CrossRef

Identification of essential genes of Pseudomonas aeruginosa for its growth in airway mucus: Mohammed Abd Alrahman , Sang Sun Yoon; J. Microbiol. 2017;55(1):68-74. Published online December 30, 2016; DOI: https://doi.org/10.1007/s12275-017-6515-3

21 View
0 Download
8 Crossref

Abstract

Pseudomonas aeruginosa has been identified as an important causative agent of airway infection, mainly in cystic fibrosis. This disease is characterized by defective mucociliary clearance induced in part by mucus hyper-production. Mucin is a major component of airway mucus and is heavily O-glycosylated, with a protein backbone. Airway infection is known to be established with bacterial adhesion to mucin. However, the genes involved in mucin degradation or utilization remain elusive. In this study, we sought to provide a genetic basis of P. aeruginosa airway growth by identifying those genes. First, using RNASeq analyses, we compared genome-wide expression profiles of PAO1, a prototype P. aeruginosa laboratory strain, grown in M9-mucin (M9M) and M9-glucose (M9G) media. Additionally, a PAO1 transposon (Tn) insertion mutants library was screened for mutants defective in growth in M9M medium. One mutant with a Tn insertion in the xcpU gene (PA3100) was determined to exhibit faulty growth in M9M medium. This gene contributes to the type II secretion system, suggesting that P. aeruginosa uses this secretion system to produce a number of proteins to break down and assimilate the mucin molecule. Furthermore, we screened the PAO1 genome for genes with protease activity. Of 13 mutants, one with mutation in PA3247 gene exhibited defective growth in M9M, suggesting that the PA3247-encoded protease plays a role in mucin utilization. Further mechanistic dissection of this particular process will reveal new drug targets, the inhibition of which could control recalcitrant P. aeruginosa infections.

Citations

Citations to this article as recorded by

Citrobacter rodentium possesses a functional type II secretion system necessary for successful host infection
Z Krekhno, SE Woodward, A Serapio-Palacios, J Peña-Díaz, KM Moon, LJ Foster, BB Finlay
Gut Microbes.2024;[Epub] CrossRef
Cross-talk between cancer and Pseudomonas aeruginosa mediates tumor suppression
Juliana K. Choi, Samer A. Naffouje, Masahide Goto, Jing Wang, Konstantin Christov, David J. Rademacher, Albert Green, Arlene A. Stecenko, Ananda M. Chakrabarty, Tapas K. Das Gupta, Tohru Yamada
Communications Biology.2023;[Epub] CrossRef
Pseudomonas aeruginosa type IV pili actively induce mucus contraction to form biofilms in tissue-engineered human airways
Tamara Rossy, Tania Distler, Lucas A. Meirelles, Joern Pezoldt, Jaemin Kim, Lorenzo Talà, Nikolaos Bouklas, Bart Deplancke, Alexandre Persat, Victor Sourjik
PLOS Biology.2023; 21(8): e3002209. CrossRef
Impact of diet and the bacterial microbiome on the mucous barrier and immune disorders
Charlotte A. Alemao, Kurtis F. Budden, Henry M. Gomez, Saima F. Rehman, Jacqueline E. Marshall, Shakti D. Shukla, Chantal Donovan, Samuel C. Forster, Ian A. Yang, Simon Keely, Elizabeth R. Mann, Emad M. El Omar, Gabrielle T. Belz, Philip M. Hansbro
Allergy.2021; 76(3): 714. CrossRef
The Bactericidal Tandem Drug, AB569: How to Eradicate Antibiotic-Resistant Biofilm Pseudomonas aeruginosa in Multiple Disease Settings Including Cystic Fibrosis, Burns/Wounds and Urinary Tract Infections
Daniel J. Hassett, Rhett A. Kovall, Michael J. Schurr, Nalinikanth Kotagiri, Harshita Kumari, Latha Satish
Frontiers in Microbiology.2021;[Epub] CrossRef
Structural and functional analysis of the carotenoid biosynthesis genes of aPseudomonasstrain isolated from the excrement of Autumn Darter
Yuki Fukaya, Miho Takemura, Takashi Koyanagi, Takashi Maoka, Kazutoshi Shindo, Norihiko Misawa
Bioscience, Biotechnology, and Biochemistry.2018; 82(6): 1043. CrossRef
Evolutionary conservation of the antimicrobial function of mucus: a first defence against infection
Cassie R Bakshani, Ana L Morales-Garcia, Mike Althaus, Matthew D Wilcox, Jeffrey P Pearson, John C Bythell, J Grant Burgess
npj Biofilms and Microbiomes.2018;[Epub] CrossRef
Expanding Role of Type II Secretion in Bacterial Pathogenesis and Beyond
Nicholas P. Cianciotto, Richard C. White, Anthony T. Maurelli
Infection and Immunity.2017;[Epub] CrossRef

Review

Against friend and foe: Type 6 effectors in plant-associated bacteria: Choong-Min Ryu; J. Microbiol. 2015;53(3):201-208. Published online March 3, 2015; DOI: https://doi.org/10.1007/s12275-015-5055-y

23 View
0 Download
31 Crossref

Abstract

Bacterial secretion systems play critical roles in communication with neighboring bacteria and in the modulation of host immune responses via the secretion of small proteins called effectors. Several secretion systems have been identified and these are denoted types I-II. Of these, the type VI secretion system (T6SS) and its effectors were only recently elucidated. Most studies on the role and significance of the T6SS and its effectors have focused on human pathogens. In this review, type 6 effectors from plant-associated beneficial and pathogenic bacteria are discussed, including effectors from Agrobacterium tumefaciens, Dickeya dadanti, Rhizobium leguminosarum, Pectobacterium atroseptium, Ralstonia solanacearum, Pseudomonas syringae, Pseudomonas fluorescens, and Pseudomonas protegens. Type 6 effectors act in symbiosis, biofilm formation, virulence, and interbacterial competition. Understanding the impact of type 6 effectors on pathogenesis will contribute to the management of bacterial pathogens in crop plants by allowing the manipulation of intra and inter-specific interactions.

Citations

Citations to this article as recorded by

Adapting the inoculation methods of kiwifruit canker disease to identify efficient biocontrol bacteria from branch microbiome
Xiaolong Shao, Qianhua Wu, Li Li, Weimei He, Xueting He, Dongjin Cheng, Aprodisia Murero, Long Lin, Limin Wang, Caihong Zhong, Lili Huang, Guoliang Qian
Molecular Plant Pathology.2024;[Epub] CrossRef
Identification of non‐canonical antagonistic bacteria via interspecies contact‐dependent killing
Long Lin, Min Tao, Wei‐Mei He, Qian‐Hua Wu, Hao‐Kai Huang, Aprodisia Kavutu Murero, Xiao‐Long Shao, Li‐Min Wang, Guo‐Liang Qian
Pest Management Science.2024; 80(8): 3997. CrossRef
Microbiota in a long survival discourse with the human host
Joseph A. Ayariga, Iddrisu Ibrahim, Logan Gildea, James Abugri, Robert Villafane
Archives of Microbiology.2023;[Epub] CrossRef
T6SS: A Key to Pseudomonas’s Success in Biocontrol?
Edwin D. Navarro-Monserrat, Christopher G. Taylor
Microorganisms.2023; 11(11): 2718. CrossRef
Disruption of the metC Gene Affects Methionine Biosynthesis in Pectobacterium carotovorum subsp. carotovorum Pcc21 and Reduces Soft-Rot Disease
Seonmi Yu, Jihee Kang, Eui-Hwan Chung, Yunho Lee
The Plant Pathology Journal.2023; 39(1): 62. CrossRef
Participation of type VI secretion system in plant colonization of phosphate solubilizing bacteria
Cinthia Tamara Lucero, Graciela Susana Lorda, Liliana Mercedes Ludueña, Fiorela Nievas, Pablo Cesar Bogino, Jorge Angelini, Mariela Lis Ambrosino, Tania Taurian
Rhizosphere.2022; 24: 100582. CrossRef
The Bradyrhizobium Sp. LmicA16 Type VI Secretion System Is Required for Efficient Nodulation of Lupinus Spp.
L. Tighilt, F. Boulila, B. F. S. De Sousa, E. Giraud, T. Ruiz-Argüeso, J. M. Palacios, J. Imperial, L. Rey
Microbial Ecology.2022; 84(3): 844. CrossRef
High-resolution spatial and genomic characterization of coral-associated microbial aggregates in the coral Stylophora pistillata
Naohisa Wada, Ming-Tsung Hsu, Kshitij Tandon, Silver Sung-Yun Hsiao, Hsing-Ju Chen, Yu-Hsiang Chen, Pei-Wen Chiang, Sheng-Ping Yu, Chih-Ying Lu, Yu-Jing Chiou, Yung-Chi Tu, Xuejiao Tian, Bi-Chang Chen, Der-Chuen Lee, Hideyuki Yamashiro, David G. Bourne, S
Science Advances.2022;[Epub] CrossRef
Agronomic efficiency and genome mining analysis of the wheat-biostimulant rhizospheric bacterium Pseudomonas pergaminensis sp. nov. strain 1008T
Marisa Díaz, Teresa Bach, Gustavo González Anta, Betina Agaras, Daniel Wibberg, Fabián Noguera, Wilter Canciani, Claudio Valverde
Frontiers in Plant Science.2022;[Epub] CrossRef
Auxin Plays Multiple Roles during Plant–Pathogen Interactions
Barbara N. Kunkel, Joshua M.B. Johnson
Cold Spring Harbor Perspectives in Biology.2021; 13(9): a040022. CrossRef
A Call for Research: A Resource of Core Microbial Symbionts of the Arabidopsis thaliana Microbiome Ready and Awaiting Experimental Exploration
Kenneth Dumack, Melanie Sapp, Tiemo von Steimker, Anna Tatjana Mänz, Laura Ellen Rose, Michael Bonkowski
Phytobiomes Journal.2021; 5(3): 362. CrossRef
Reciprocal adaptation of rice and Xanthomonas oryzae pv. oryzae: cross-species 2D GWAS reveals the underlying genetics
Fan Zhang, Zhiqiang Hu, Zhichao Wu, Jialing Lu, Yingyao Shi, Jianlong Xu, Xiyin Wang, Jinpeng Wang, Fan Zhang, Mingming Wang, Xiaorong Shi, Yanru Cui, Casiana Vera Cruz, Dalong Zhuo, Dandan Hu, Min Li, Wensheng Wang, Xiuqin Zhao, Tianqing Zheng, Binying F
The Plant Cell.2021; 33(8): 2538. CrossRef
Full Issue PDF

Phytobiomes Journal.2021; 5(3): 249. CrossRef
The Azospirillum brasilense type VI secretion system promotes cell aggregation, biocontrol protection against phytopathogens and attachment to the microalgae Chlorella sorokiniana
Fabricio D. Cassan, Anahí Coniglio, Edgar Amavizca, Guillermo Maroniche, Eric Cascales, Yoav Bashan, Luz E. de‐Bashan
Environmental Microbiology.2021; 23(10): 6257. CrossRef
Protective role of the Arabidopsis leaf microbiota against a bacterial pathogen
Christine M. Vogel, Daniel B. Potthoff, Martin Schäfer, Niculò Barandun, Julia A. Vorholt
Nature Microbiology.2021; 6(12): 1537. CrossRef
Xenorhabdus bovienii strain jolietti uses a type 6 secretion system to kill closely related Xenorhabdus strains
Rebecca M Kochanowsky, Christine Bradshaw, Isabel Forlastro, S Patricia Stock
FEMS Microbiology Ecology.2020;[Epub] CrossRef
Secretion Systems of Bacterial Phytopathogens and Mutualists (Review)
L. A. Lomovatskaya, A. S. Romanenko
Applied Biochemistry and Microbiology.2020; 56(2): 115. CrossRef
Two Functionally Deviating Type 6 Secretion Systems Occur in the Nitrogen-Fixing Endophyte Azoarcus olearius BH72
Xun Jiang, Andreas Beust, Praveen K. Sappa, Uwe Völker, Theresa Dinse, Julia Herglotz, Barbara Reinhold-Hurek
Frontiers in Microbiology.2019;[Epub] CrossRef
The phytopathogenic nature of Dickeya aquatica 174/2 and the dynamic early evolution of Dickeya pathogenicity
Alexandre Duprey, Najwa Taib, Simon Leonard, Tiffany Garin, Jean‐Pierre Flandrois, William Nasser, Céline Brochier‐Armanet, Sylvie Reverchon
Environmental Microbiology.2019; 21(8): 2809. CrossRef
Bioinformatic Analysis of the Type VI Secretion System and Its Potential Toxins in the Acinetobacter Genus
Guillermo D. Repizo, Martín Espariz, Joana L. Seravalle, Suzana P. Salcedo
Frontiers in Microbiology.2019;[Epub] CrossRef
The Type VI secretion system of Rhizobium etli Mim1 has a positive effect in symbiosis
Alvaro Salinero-Lanzarote, Alba Pacheco-Moreno, Lucía Domingo-Serrano, David Durán, Ernesto Ormeño-Orrillo, Esperanza Martínez-Romero, Marta Albareda, José Manuel Palacios, Luis Rey
FEMS Microbiology Ecology.2019;[Epub] CrossRef
Convergent Evolution in Intracellular Elements: Plasmids as Model Endosymbionts
Anne-Kathrin Dietel, Martin Kaltenpoth, Christian Kost
Trends in Microbiology.2018; 26(9): 755. CrossRef
In vivo TssA proximity labelling during type VI secretion biogenesis reveals TagA as a protein that stops and holds the sheath
Yoann G. Santin, Thierry Doan, Régine Lebrun, Leon Espinosa, Laure Journet, Eric Cascales
Nature Microbiology.2018; 3(11): 1304. CrossRef
The roles of auxin during interactions between bacterial plant pathogens and their hosts
Barbara N Kunkel, Christopher P Harper
Journal of Experimental Botany.2018; 69(2): 245. CrossRef
Genome-Wide Analysis of Type VI System Clusters and Effectors in Burkholderia Species
Thao Thi Nguyen, Hyun-Hee Lee, Inmyoung Park, Young-Su Seo
The Plant Pathology Journal.2018; 34(1): 11. CrossRef
An Alternative Nested Reading Frame May Participate in the Stress-Dependent Expression of a Plant Gene
Ekaterina V. Sheshukova, Tatiana V. Komarova, Natalia M. Ershova, Anastasia V. Shindyapina, Yuri L. Dorokhov
Frontiers in Plant Science.2017;[Epub] CrossRef
Role of the type VI secretion systems during disease interactions of Erwinia amylovora with its plant host
Tim Kamber, Joël F. Pothier, Cosima Pelludat, Fabio Rezzonico, Brion Duffy, Theo H. M. Smits
BMC Genomics.2017;[Epub] CrossRef
Mutations in Two Paraburkholderia phymatum Type VI Secretion Systems Cause Reduced Fitness in Interbacterial Competition
Samanta Bolzan de Campos, Martina Lardi, Alessia Gandolfi, Leo Eberl, Gabriella Pessi
Frontiers in Microbiology.2017;[Epub] CrossRef
Metal homeostasis in bacteria: the role of ArsR–SmtB family of transcriptional repressors in combating varying metal concentrations in the environment
Rudra P. Saha, Saikat Samanta, Surajit Patra, Diganta Sarkar, Abinit Saha, Manoj Kumar Singh
BioMetals.2017; 30(4): 459. CrossRef
Toxin-Antitoxin Systems: Implications for Plant Disease
T. Shidore, L.R. Triplett
Annual Review of Phytopathology.2017; 55(1): 161. CrossRef
The Plant Microbiota: Systems-Level Insights and Perspectives
Daniel B. Müller, Christine Vogel, Yang Bai, Julia A. Vorholt
Annual Review of Genetics.2016; 50(1): 211. CrossRef

Research Support, Non-U.S. Gov'ts

Crystal structure of the bacterial type VI secretion system component TssL from Vibrio cholerae: Jeong Ho Chang , Yeon-Gil Kim; J. Microbiol. 2015;53(1):32-37. Published online December 4, 2014; DOI: https://doi.org/10.1007/s12275-015-4539-0

22 View
0 Download
10 Crossref

Abstract

The type VI secretion system (T6SS), commonly found in Gram-negative bacteria, is responsible for exporting effector proteins. The T6SS has been reported to be cytotoxic to host cells. While the components and assembly of the T6SS complex have been largely assessed, structural data on T6SS components from virulent bacteria is remarkably insufficient. Here, we report the crystal structure of Vibrio cholerae TssL (VcTssL), a core component of T6SS. In spite of a relatively low sequence identity, the overall structure of VcTssL is largely similar to those from other bacterial homologs except for several differences found in local structural elements. A unique feature attributed to the C-terminal fragment of Vc- TssL is a crystallographic artifact. This incidental feature of VcTssL may provide insights into screening of molecular partners for the cytoplasmic domain of TssL. Additionally, our results may help in the design of molecular probes for a detailed understanding of the functional relationship between TssL and other T6SS components.

Citations

Citations to this article as recorded by

Structural Characterization of TssL from Acinetobacter baumannii: a Key Component of the Type VI Secretion System
Federico M. Ruiz, Juvenal Lopez, C. Gastón Ferrara, Elena Santillana, Yanis R. Espinosa, Mario F. Feldman, Antonio Romero, Ann M. Stock
Journal of Bacteriology.2020;[Epub] CrossRef
In situ and high‐resolution cryo‐ EM structure of a bacterial type VI secretion system membrane complex
Chiara Rapisarda, Yassine Cherrak, Romain Kooger, Victoria Schmidt, Riccardo Pellarin, Laureen Logger, Eric Cascales, Martin Pilhofer, Eric Durand, Rémi Fronzes
The EMBO Journal.2019;[Epub] CrossRef
Crystal Structure of the Type VI Secretion System Accessory Protein TagF from Pseudomonas Aeruginosa
Chang-Kyu Ok, Jeong Ho Chang
Protein & Peptide Letters.2019; 26(3): 204. CrossRef
Structure and Activity of the Type VI Secretion System
Yassine Cherrak, Nicolas Flaugnatti, Eric Durand, Laure Journet, Eric Cascales, Maria Sandkvist, Peter J. Christie
Microbiology Spectrum.2019;[Epub] CrossRef
Crystal structure of the periplasmic domain of TssL, a key membrane component of Type VI secretion system
Xiangbei Wang, Bo Sun, Mengxue Xu, Shenshen Qiu, Dongqing Xu, Tingting Ran, Jianhua He, Weiwu Wang
International Journal of Biological Macromolecules.2018; 120: 1474. CrossRef
Tryptophan-mediated Dimerization of the TssL Transmembrane Anchor Is Required for Type VI Secretion System Activity
Abdelrahim Zoued, Jean-Pierre Duneau, Eric Durand, Alexandre P. España, Laure Journet, Françoise Guerlesquin, Eric Cascales
Journal of Molecular Biology.2018; 430(7): 987. CrossRef
Structure–Function Analysis of the TssL Cytoplasmic Domain Reveals a New Interaction between the Type VI Secretion Baseplate and Membrane Complexes
Abdelrahim Zoued, Chloé J. Cassaro, Eric Durand, Badreddine Douzi, Alexandre P. España, Christian Cambillau, Laure Journet, Eric Cascales
Journal of Molecular Biology.2016; 428(22): 4413. CrossRef
Aim, Load, Fire: The Type VI Secretion System, a Bacterial Nanoweapon
Francesca R. Cianfanelli, Laura Monlezun, Sarah J. Coulthurst
Trends in Microbiology.2016; 24(1): 51. CrossRef
Biogenesis and structure of a type VI secretion membrane core complex
Eric Durand, Van Son Nguyen, Abdelrahim Zoued, Laureen Logger, Gérard Péhau-Arnaudet, Marie-Stéphanie Aschtgen, Silvia Spinelli, Aline Desmyter, Benjamin Bardiaux, Annick Dujeancourt, Alain Roussel, Christian Cambillau, Eric Cascales, Rémi Fronzes
Nature.2015; 523(7562): 555. CrossRef
Type VI secretion system: secretion by a contractile nanomachine
Marek Basler
Philosophical Transactions of the Royal Society B: Biological Sciences.2015; 370(1679): 20150021. CrossRef

NOTE] Comparative Assessment of the Intracellular Survival of the Burkholderia pseudomallei bopC Mutant: Varintip Srinon , Sunsiree Muangman , Nithima Imyaem , Veerachat Muangsombut , Natalie R. Lazar Adler , Edouard E. Galyov , Sunee Korbsrisate; J. Microbiol. 2013;51(4):522-526. Published online August 30, 2013; DOI: https://doi.org/10.1007/s12275-013-2557-3

13 View
0 Download
14 Scopus

Abstract: Burkholderia pseudomallei, the causative agent of melioidosis, is a Gram-negative saprophytic bacterium capable of surviving within phagocytic cells. To assess the role of BopC (a type III secreted effector protein) in the pathogenesis of B. pseudomallei, a B. pseudomallei bopC mutant was used to infect J774A.1 macrophage-like cells. The bopC mutant showed significantly reduced intracellular survival in infected macrophages compared to wild-type B. pseudomallei. In addition, the bopC mutant displayed delayed escape from endocytic vesicles compared with the wild-type strain. This indicates that BopC is important, and at least in part, needed for intracellular survival of B. pseudomallei.

The α-Barrel Tip Region of Escherichia coli TolC Homologs of Vibrio vulnificus Interacts with the MacA Protein to Form the Functional Macrolide-Specific Efflux Pump MacAB-TolC: Minho Lee , Hyun-Lee Kim , Saemee Song , Minju Joo , Seunghwa Lee , Daeyoung Kim , Yoonsoo Hahn , Nam-Chul Ha , Kangseok Lee; J. Microbiol. 2013;51(2):154-159. Published online April 27, 2013; DOI: https://doi.org/10.1007/s12275-013-2699-3

23 View
0 Download
18 Scopus

Abstract: TolC and its homologous family of proteins are outer membrane factors that are essential for exporting small molecules and toxins across the outer membrane in Gram-negative bacteria. Two open reading frames in the Vibrio vulnificus genome that encode proteins homologous to Escherichia coli TolC, designated TolCV1 and TolCV2, have 51.3% and 29.6% amino acid identity to TolC, respectively. In this study, we show that TolCV1 and TolCV2 functionally and physically interacted with the membrane fusion protein, MacA, a component of the macrolide-specific MacAB-TolC pump of E. coli. We further show that the conserved residues located at the aperture tip region of the α-hairpin of TolCV1 and TolCV2 played an essential role in the formation of the functional MacAB-TolC pump using site-directed mutational analyses. Our findings suggest that these outer membrane factors have conserved tip-to-tip interaction with the MacA membrane fusion protein for action of the drug efflux pump in Gramnegative bacteria.

Secretion of Truncated Recombinant Rabies Virus Glycoprotein with Preserved Antigenic Properties Using a Co-Expression System in Hansenula polymorpha: Weidong Qian , Frank Aguilar , Ting Wang , Bingsheng Qiu; J. Microbiol. 2013;51(2):234-240. Published online April 27, 2013; DOI: https://doi.org/10.1007/s12275-013-2337-0

25 View
0 Download
11 Scopus

Abstract: Rabies virus infection remains a serious public health threat in the developing world, where cost-concerns make widescale public health interventions impractical. The development of novel and inexpensive ELISA diagnostic antigens is critical in early detection and prevention of complications. The transmembrane glycoprotein (G) of rabies virus (RV) contains an external domain capable of inducing the synthesis of anti-rabies, virus-neutralizing antibodies, in infected or immunized hosts. In our study, the external G domain was synthesized and fused in-frame with a polyhistidine-tag coding sequence present in the expression plasmid. Soluble truncated recombinant G was secreted in Hansenula polymorpha (H. polymorpha) using H. polymorpha-derived calnexin (HpCNE1) overproduction and found to be correctly N-glycosylated. The truncated recombinant G was purified from cell culture supernatant by Ni-agarose affinity chromatography and when compared with the full-length glycoprotein, found to be similarly immunogenic in vaccinated rabbits. These results subsequently led us to explore the potential of truncated recombinant G as a diagnostic antigen in ELISA. Our results show that the truncated recombinant G can detect antibodies directed to both whole virion and native glycoprotein. More sophisticated applications of truncated recombinant G would profit from the correctly N-glycosylated and soluble monomer.

Review

REVIEW] The Role of Type III Secretion System 2 in Vibrio parahaemolyticus Pathogenicity: Hyeilin Ham , Kim Orth; J. Microbiol. 2012;50(5):719-725. Published online November 4, 2012; DOI: https://doi.org/10.1007/s12275-012-2550-2

18 View
0 Download
46 Scopus

Abstract: Vibrio parahaemolyticus, a Gram-negative marine bacterial pathogen, is emerging as a major cause of food-borne illnesses worldwide due to the consumption of raw seafood leading to diseases including gastroenteritis, wound infection, and septicemia. The bacteria utilize toxins and type III secretion system (T3SS) to trigger virulence. T3SS is a multi-subunit needle-like apparatus used to deliver bacterial proteins, termed effectors, into the host cytoplasm which then target various eukaryotic signaling pathways. V. parahaemolyticus carries two T3SSs in each of its two chromosomes, named T3SS1 and T3SS2, both of which play crucial yet distinct roles during infection: T3SS1 causes cytotoxicity whereas T3SS2 is mainly associated with enterotoxicity. Each T3SS secretes a unique set of effectors that contribute to virulence by acting on different host targets and serving different functions. Emerging studies on T3SS2 of V. parahaemolyticus, reveal its regulation, translocation, discovery, characterization of its effectors, and development of animal models to understand the enterotoxicity. This review on recent findings for T3SS2 of V. parahaemolyticus highlights a novel mechanism of invasion that appears to be conserved by other marine bacteria.

Journal Article

Disruption of SCO5461 Gene Coding for a Mono-ADP-Ribosyltransferase Enzyme Produces a Conditional Pleiotropic Phenotype Affecting Morphological Differentiation and Antibiotic Production in Streptomyces coelicolor: Krisztina Szirák , Judit Keser&# , Sándor Biró , Iván Schmelczer , György Barabás , András Penyige; J. Microbiol. 2012;50(3):409-418. Published online June 30, 2012; DOI: https://doi.org/10.1007/s12275-012-1440-y

15 View
0 Download
9 Scopus

Abstract: The SCO5461 gene of Streptomyces coelicolor A3(2) codes for an ADP-ribosyltransferase enzyme that is predicted to be a transmembrane protein with an extracellular catalytic domain. PCR-targeted disruption of the gene resulted in a mutant that differentiated normally on complex SFM medium; however, morphological differentiation in minimal medium was significantly delayed and this phenotype was even more pronounced on osmotically enhanced minimal medium. The mutant did not sporulate when it was grown on R5 medium, however the normal morphological differentiation was restored when the strain was cultivated beside the wild-type S. coelicolor M145 strain. Comparison of the pattern of ADP-ribosylated proteins showed a difference between the mutant and the wild type, fewer modified proteins were present in the cellular crude extract of the mutant strain. These results support our previous suggestions that protein ADP-ribosylation is involved in the regulation of differentiation and antibiotic production and secretion in Streptomyces.

Research Support, Non-U.S. Gov'ts

Effect of Acidic pH on the Invasion Efficiency and the Type III Secretion System of Burkholderia thailandensis: Siroj Jitprasutwit , Wisansanee Thaewpia , Veerachat Muangsombut , Aroonlug Lulitanond , Chanvit Leelayuwat , Ganjana Lertmemongkolchai , Sunee Korbsrisate; J. Microbiol. 2010;48(4):526-532. Published online August 20, 2010; DOI: https://doi.org/10.1007/s12275-010-0078-x

14 View
0 Download
9 Scopus

Abstract: Burkholderia thailandensis is a close relative of Burkholderia pseudomallei. These organisms are very similar, but B. thailandensis is far less virulent than B. pseudomallei. Nucleotide sequencing and analysis of 14 B. thailandensis isolates revealed variation in the regions coding for the type III secreted BipD protein. The degree of B. thailandensis BipD sequence variation was greater than that found in B. pseudomallei. Western blot analysis indicated that, unlike B. pseudomallei, B. thailandensis type III secreted proteins including BipD and BopE could not be detected in the supernatant of culture medium unless induced by acidic conditions. In addition, culturing B. thailandensis under acidic growth conditions (pH 4.5) can induce the ability of this bacterium to invade human respiratory epithelial cells A549. The identification of an environmental stimulus that increases the invasion capability of B. thailandensis invasion is of value for those who would like to use this bacterium as a model to study B. pseudomallei virulence.

Stable Expression and Secretion of Polyhydroxybutyrate Depolymerase of Paucimonas lemoignei in Escherichia coli: Se Whan Park , Moon Gyu Chung , Hwa Young Lee , Jeong Yoon Kim , Young Ha Rhee; J. Microbiol. 2008;46(6):662-669. Published online December 24, 2008; DOI: https://doi.org/10.1007/s12275-008-0283-z

18 View
0 Download
1 Scopus

Abstract: An efficient strategy for the expression and secretion of extracellular polyhydroxybutyrate depolymerase (PhaZ1) of Paucimonas lemoignei in Escherichia coli was developed by employing the signal peptide of PhaZ1 and a truncated ice nucleation protein anchoring motif (INPNC). Directly synthesized mature form of PhaZ1 was present in the cytoplasm of host cells as inclusion bodies, while a construct containing PhaZ1 and its own N-terminal signal peptide (PrePhaZ1) enabled the secretion of active PhaZ1 into the extracellular medium. However, the PrePhaZ1 construct was harmful to the host cell and resulted in atypical growth and instability of the plasmid during the cultivation. In contrast, INPNC-PhaZ1 and INPNCPrePhaZ1 fusion constructs did not affect growth of host cells. INPNC-PhaZ1 was successfully displayed on the cell surface with its fusion form, but did not retain PhaZ1 activity. In the case of INPNC-PrePhaZ1, the initially synthesized fusion form was separated by precise cleavage of the signal peptide, and active PhaZ1 was consequently released into the culture medium. The amount of PhaZ1 derived from E. coli (INPNC-PrePhaZ1) was almost twice as great as that directly expressed from E. coli (PrePhaZ1), and was predominantly (approximately 85%) located in the periplasm when cultivated at 22°C but was efficiently secreted into the extracellular medium when cultivated at 37°C.

First
Prev
Page of 2
Next
Last

Search