Journal of Microbiology

Full article

Mouse strain-dependent neutralizing antibody responses to Zika virus vaccines: Sang Hwan Seo, Jung-ah Choi, Eunji Yang, Hayan Park, Dae-Im Jung, Jae-Ouk Kim, Jae Seung Yang, Manki Song; J. Microbiol. 2025;63(8):e2504005. Published online August 31, 2025; DOI: https://doi.org/10.71150/jm.2504005

1,198 View
18 Download

Abstract PDF: The 2015 Zika virus (ZIKV) outbreak in Brazil and its global spread underscored the urgent need for effective and broadly protective vaccines. While C57BL/6 and BALB/c mice are widely used in preclinical vaccine research, direct comparisons of their ability to elicit ZIKV-specific neutralizing antibodies (nAbs) remain limited. This study aimed to systematically evaluate and compare the immunogenic potential of these two common mouse strains across diverse vaccine platforms, focusing on their capacity to generate functional neutralizing antibody responses. We assessed nAb and IgG responses following four vaccination strategies: (1) DNA vaccine encoding prMEΔTM followed by E protein domain III boost, (2) recombinant EΔTM protein expressed using baculovirus system, (3) formalin-inactivated ZIKV, and (4) live ZIKV. Although both strains generated detectable ZIKV- and E protein-specific IgG, the magnitude and quality of responses varied by vaccine platform and strain. Notably, C57BL/6 mice consistently mounted significantly higher nAb titers than BALB/c mice across all immunization groups, including subunit- and whole-virus-based vaccines. In contrast, BALB/c mice showed lower or undetectable nAb responses, despite comparable or higher total IgG levels in some cases. These findings show that host genetic background is a critical determinant of vaccine-induced neutralization and underscore the importance of selecting appropriate animal models in ZIKV vaccine development. C57BL/6 mice, due to their robust nAb responses, represent a reliable model for evaluating vaccine immunogenicity. Conversely, the limited nAb responses in BALB/c mice position them as a potential low-responder model, offering a stringent system to test the potency and breadth of protective immunity under suboptimal conditions.

Reviews

Advancements in dengue vaccines: A historical overview and pro-spects for following next-generation candidates: Kai Yan, Lingjing Mao, Jiaming Lan, Zhongdang Xiao; J. Microbiol. 2025;63(2):e2410018. Published online February 27, 2025; DOI: https://doi.org/10.71150/jm.2410018

9,580 View
392 Download
2 Web of Science
3 Crossref

Dengue, caused by four serotypes of dengue viruses (DENV-1 to DENV-4), is the most prevalent and widely mosquito-borne viral disease affecting humans. Dengue virus (DENV) infection has been reported in over 100 countries, and approximately half of the world's population is now at risk. The paucity of universally licensed DENV vaccines highlights the urgent need to address this public health concern. Action and attention to antibody-dependent enhancement increase the difficulty of vaccine development. With the worsening dengue fever epidemic, Dengvaxia^® (CYD-TDV) and Qdenga^® (TAK-003) have been approved for use in specific populations in affected areas. However, these vaccines do not provide a balanced immune response to all four DENV serotypes and the vaccination cannot cover all populations. There is still a need to develop a safe, broad-spectrum, and effective vaccine to address the increasing number of dengue cases worldwide. This review provides an overview of the existing DENV vaccines, as well as potential candidates for future studies on DENV vaccine development, and discusses the challenges and possible solutions in the field.

Citations

Citations to this article as recorded by

Role of c-ABL in DENV-2 Infection and Actin Remodeling in Vero Cells
Grace Paola Carreño-Flórez, Alexandra Milena Cuartas-López, Ryan L. Boudreau, Miguel Vicente-Manzanares, Juan Carlos Gallego-Gómez
International Journal of Molecular Sciences.2025; 26(9): 4206. CrossRef
Crystallographic Fragment Screening of the Dengue Virus Polymerase Reveals Multiple Binding Sites for the Development of Non-nucleoside Antiflavivirals
Manisha Saini, Jasmin C. Aschenbrenner, Francesc Xavier Ruiz, Ashima Chopra, Anu V. Chandran, Peter G. Marples, Blake H. Balcomb, Daren Fearon, Frank von Delft, Eddy Arnold
Journal of Medicinal Chemistry.2025; 68(17): 18356. CrossRef
Understanding the Diversity of Dengue Serotypes: Impacts on Public Health and Disease Control
Gopinath Ramalingam, Madhumitha Patchaiyappan, M. Arundadhi, Krishnapriya Subramani, A. Dhanasezhian, Sucila Thangam Ganesan
The Journal of Medical Research.2025; 11(4): 69. CrossRef

Recent Advances of Nipah Virus Disease: Pathobiology to Treatment and Vaccine Advancement: Sagnik Saha, Manojit Bhattacharya, Sang-Soo Lee, Chiranjib Chakraborty; J. Microbiol. 2024;62(10):811-828. Published online September 18, 2024; DOI: https://doi.org/10.1007/s12275-024-00168-3

237 View
6 Download
2 Web of Science
3 Crossref

Abstract PDF

The zoonotic infection of the Nipah virus (NiV) has yet again appeared in 2023 in Kerala state, India. The virus, which has a mortality rate ranging from about 40 to 70%, has already infected India five times, the first being in 2001. The current infection is the sixth virus outbreak in the Indian population. In 1998, the first NiV infection was noted in one village in Malaysia. After that, outbreaks from other South and Southeast Asian countries have been reported periodically. It can spread between humans through contact with body fluids. Therefore, it is unlikely to generate a new pandemic. However, there is a considerable knowledge gap in the different areas of NiV. To date, no approved vaccines or treatments have been available. To fulfil the knowledge gap, the review article provided a detailed overview of the genome and genome-encoded proteins, epidemiology, transmission, pathobiology, immunobiology, diagnosis, prevention and control measures, therapeutics (monoclonal antibodies and drug molecules), and vaccine advancement of the emerging and deadly pathogen. The advanced information will help researchers to develop safe and effective NiV vaccine and treatment regimens worldwide.

Citations

Citations to this article as recorded by

An Overview of Nipah Virus Infection
Ujjawal Singh, Ramsha Sharma, Raj Kamal, Ranjeet Kumar
Anti-Infective Agents.2025;[Epub] CrossRef
Antiviral effects of heme oxygenase-1 against canine coronavirus and canine influenza virus in vitro
Jae-Hyeong Kim, Dong-Hwi Kim, Kyu-Beom Lim, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Dong-Hun Lee, Do-Geun Kim, Hun-Young Yoon, In-Soo Choi
Journal of Microbiology.2025; 63(5): e2501029. CrossRef
Efficient and modular reverse genetics system for rapid generation of recombinant severe acute respiratory syndrome coronavirus 2
Sojung Bae, Jinjong Myoung
Journal of Microbiology.2025; 63(7): e2504015. CrossRef

Adenoviral Vector System: A Comprehensive Overview of Constructions, Therapeutic Applications and Host Responses: Anyeseu Park, Jeong Yoon Lee; J. Microbiol. 2024;62(7):491-509. Published online July 22, 2024; DOI: https://doi.org/10.1007/s12275-024-00159-4

555 View
9 Download
11 Web of Science
11 Crossref

Abstract PDF

Adenoviral vectors are crucial for gene therapy and vaccine development, offering a platform for gene delivery into host cells. Since the discovery of adenoviruses, first-generation vectors with limited capacity have evolved to third-generation vectors flacking viral coding sequences, balancing safety and gene-carrying capacity. The applications of adenoviral vectors for gene therapy and anti-viral treatments have expanded through the use of in vitro ligation and homologous recombination, along with gene editing advancements such as CRISPR-Cas9. Current research aims to maintain the efficacy and safety of adenoviral vectors by addressing challenges such as pre-existing immunity against adenoviral vectors and developing new adenoviral vectors from rare adenovirus types and non-human species. In summary, adenoviral vectors have great potential in gene therapy and vaccine development. Through continuous research and technological advancements, these vectors are expected to lead to the development of safer and more effective treatments.

Citations

Citations to this article as recorded by

Engineering an oncolytic adenoviral platform for precise delivery of antisense peptide nucleic acid to modulate PD-L1 overexpression in cancer cells
Andrea Patrizia Falanga, Francesca Greco, Monica Terracciano, Stefano D’Errico, Maria Marzano, Sara Feola, Valentina Sepe, Flavia Fontana, Ilaria Piccialli, Vincenzo Cerullo, Hélder A. Santos, Nicola Borbone
International Journal of Pharmaceutics.2025; 668: 124941. CrossRef
Enhancing precision in cancer treatment: the role of gene therapy and immune modulation in oncology
Emile Youssef, Brandon Fletcher, Dannelle Palmer
Frontiers in Medicine.2025;[Epub] CrossRef
Protein-Based Degraders: From Chemical Biology Tools to Neo-Therapeutics
Lisha Ou, Mekedlawit T. Setegne, Jeandele Elliot, Fangfang Shen, Laura M. K. Dassama
Chemical Reviews.2025; 125(4): 2120. CrossRef
Intestinal mucus: the unsung hero in the battle against viral gastroenteritis
Waqar Saleem, Ateeqa Aslam, Mehlayl Tariq, Hans Nauwynck
Gut Pathogens.2025;[Epub] CrossRef
Chromatin structure and gene transcription of recombinant p53 adenovirus vector within host
Duo Ning, Yuqing Deng, Simon Zhongyuan Tian
Frontiers in Molecular Biosciences.2025;[Epub] CrossRef
Multi-level ROS regulation to activate innate and adaptive immune therapies
Ke-Ke Feng, Cheng-Lei Li, Yi-Fan Tu, Shi-Cheng Tian, Rui Xiong, Bai-Sheng Sa, Jing-Wei Shao
Chemical Engineering Journal.2025; 515: 163429. CrossRef
Genetically modified cell membrane proteins in tissue engineering and regenerative medicine
Yilin Bao, Yue Hu, Mengxuan Hao, Qinmeng Zhang, Guoli Yang, Zhiwei Jiang
Biofabrication.2025; 17(3): 032004. CrossRef
Surgical treatment of otogenic vertigo
Tian Yu, Xiaohong Chen
European Archives of Oto-Rhino-Laryngology.2025;[Epub] CrossRef
Chimeric Element-Regulated MRI Reporter System for Mediation of Glioma Theranostics
Qian Hu, Jie Huang, Xiangmin Zhang, Haoru Wang, Xiaoying Ni, Huiru Zhu, Jinhua Cai
Cancers.2025; 17(14): 2349. CrossRef
Molecular Engineering of Virus Tropism
Bo He, Belinda Wilson, Shih-Heng Chen, Kedar Sharma, Erica Scappini, Molly Cook, Robert Petrovich, Negin P. Martin
International Journal of Molecular Sciences.2024; 25(20): 11094. CrossRef
Antisolvent 3D Printing of Gene-Activated Scaffolds for Bone Regeneration
Andrey Vyacheslavovich Vasilyev, Irina Alekseevna Nedorubova, Viktoria Olegovna Chernomyrdina, Anastasiia Yurevna Meglei, Viktoriia Pavlovna Basina, Anton Vladimirovich Mironov, Valeriya Sergeevna Kuznetsova, Victoria Alexandrovna Sinelnikova, Olga Anatol
International Journal of Molecular Sciences.2024; 25(24): 13300. CrossRef

Journal Articles

Vaccine Development for Severe Fever with Thrombocytopenia Syndrome Virus in Dogs: Seok-Chan Park, Da-Eun Jeong, Sun-Woo Han, Joon-Seok Chae, Joo-Yong Lee, Hyun-Sook Kim, Bumseok Kim, Jun-Gu Kang; J. Microbiol. 2024;62(4):327-335. Published online April 18, 2024; DOI: https://doi.org/10.1007/s12275-024-00119-y

335 View
7 Download
5 Web of Science
4 Crossref

Abstract PDF

Severe fever with thrombocytopenia syndrome (SFTS) is a life-threatening viral zoonosis. The causative agent of this disease is the Dabie bandavirus, which is usually known as the SFTS virus (SFTSV). Although the role of vertebrates in SFTSV transmission to humans remains uncertain, some reports have suggested that dogs could potentially transmit SFTSV to humans. Consequently, preventive measures against SFTSV in dogs are urgently needed. In the present study, dogs were immunized three times at two-week intervals with formaldehyde-inactivated SFTSV with two types of adjuvants. SFTSV (KCD46) was injected into all dogs two weeks after the final immunization. Control dogs showed viremia from 2 to 4 days post infection (dpi), and displayed white pulp atrophy in the spleen, along with a high level of terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling assay (TUNEL) positive area. However, the inactivated SFTSV vaccine groups exhibited rare pathological changes and significantly reduced TUNEL positive areas in the spleen. Furthermore, SFTSV viral loads were not detected at any of the tested dpi. Our results indicate that both adjuvants can be safely used in combination with an inactivated SFTSV formulation to induce strong neutralizing antibodies. Inactivated SFTSV vaccines effectively prevent pathogenicity and viremia in dogs infected with SFTSV. In conclusion, our study highlighted the potential of inactivated SFTSV vaccination for SFTSV control in dogs.

Citations

Citations to this article as recorded by

The immunogenicity and protection efficacy evaluation of mRNA vaccine candidate for severe fever with thrombocytopenia syndrome in mice
Da-Eun Jeong, Jack Yoon, Baek Kim, Jun-Gu Kang, Abdallah M. Samy
PLOS Neglected Tropical Diseases.2025; 19(4): e0012999. CrossRef
Efficient and modular reverse genetics system for rapid generation of recombinant severe acute respiratory syndrome coronavirus 2
Sojung Bae, Jinjong Myoung
Journal of Microbiology.2025; 63(7): e2504015. CrossRef
Current status of severe fever with thrombocytopenia syndrome in China (Review)
Hao Sun, Quanman Hu, Saiwei Lu, Yanyan Yang, Li Zhang, Jinzhao Long, Yuefei Jin, Haiyan Yang, Shuaiyin Chen, Guangcai Duan
International Journal of Molecular Medicine.2025; 56(5): 1. CrossRef
Domain-Specific Impacts of Spike Protein Mutations on Infectivity and Antibody Escape in SARS-CoV-2 Omicron BA.1
Tae-Hun Kim, Sojung Bae, Jinjong Myoung
Journal of Microbiology and Biotechnology.2025;[Epub] CrossRef

Lactobacillus acidophilus KBL409 Ameliorates Atopic Dermatitis in a Mouse Model: Woon-ki Kim , You Jin Jang , SungJun Park , Sung-gyu Min , Heeun Kwon , Min Jung Jo , GwangPyo Ko; J. Microbiol. 2024;62(2):91-99. Published online February 22, 2024; DOI: https://doi.org/10.1007/s12275-024-00104-5

424 View
19 Download
10 Web of Science
10 Crossref

Abstract PDF

Atopic dermatitis (AD) is a chronic inflammatory skin disease with repeated exacerbations of eczema and pruritus. Probiotics can prevent or treat AD appropriately via modulation of immune responses and gut microbiota. In this study, we evaluated effects of Lactobacillus acidophilus (L. acidophilus) KBL409 using a house dust mite (Dermatophagoides farinae)-induced in vivo AD model. Oral administration of L. acidophilus KBL409 significantly reduced dermatitis scores and decreased infiltration of immune cells in skin tissues. L. acidophilus KBL409 reduced in serum immunoglobulin E and mRNA levels of T helper (Th)1 (Interferon-γ), Th2 (Interleukin [IL]-4, IL-5, IL-13, and IL-31), and Th17 (IL-17A) cytokines in skin tissues. The anti-inflammatory cytokine IL-10 was increased and Foxp3 expression was up-regulated in AD-induced mice with L. acidophilus KBL409. Furthermore, L. acidophilus KBL409 significantly modulated gut microbiota and concentrations of short-chain fatty acids and amino acids, which could explain its effects on AD. Our results suggest that L. acidophilus KBL409 is the potential probiotic for AD treatment by modulating of immune responses and gut microbiota of host.

Citations

Citations to this article as recorded by

The gut-skin axis: a bi-directional, microbiota-driven relationship with therapeutic potential
Maira Jimenez-Sanchez, Larissa S. Celiberto, Hyungjun Yang, Ho Pan Sham, Bruce A. Vallance
Gut Microbes.2025;[Epub] CrossRef
Probiotics ameliorate atopic dermatitis by modulating the dysbiosis of the gut microbiota in dogs
Hyokeun Song, Seung-Hyun Mun, Dae-Woong Han, Jung-Hun Kang, Jae-Uk An, Cheol-Yong Hwang, Seongbeom Cho
BMC Microbiology.2025;[Epub] CrossRef
The effect of daily oral probiotic and postbiotic supplementation on the canine skin microbiota: Insights from culture‐dependent and long‐read 16S rRNA gene sequencing methods
Letitia Grant, Manijeh Mohammadi Dehcheshmeh, Esmaeil Ebrahimie, Aliakbar Khabiri, Tania Veltman, Michael Shipstone, Darren J. Trott
Veterinary Dermatology.2025; 36(5): 581. CrossRef
The efficacy of Akkermansia muciniphila YGMCC2602-derived postbiotics in skin repair
Zhili He, Wenfang Song, Shichang Zhang, Minlei Zhao, Fan Wang, Shanshan He, Xiaochi Jie, Qi Gao, Jianguo Chen
Journal of Functional Foods.2025; 131: 106950. CrossRef
Differential modulation of post-antibiotic colonization resistance to Clostridioides difficile by two probiotic Lactobacillus strains
Matthew H. Foley, Arthur S. McMillan, Sarah O'Flaherty, Rajani Thanissery, Molly E. Vanhoy, Mary Gracen Fuller, Rodolphe Barrangou, Casey M. Theriot, Jacques Ravel
mBio.2025;[Epub] CrossRef
Innovative microbial strategies in atopic dermatitis
Jingtai Ma, Yiting Fang, Jinxing Hu, Shiqi Li, Lilian Zeng, Siyi Chen, Zhifeng Li, Ruiling Meng, Xingfen Yang, Fenglin Zhang, Guiyuan Ji, Peihua Liao, Liang Chen, Wei Wu
Frontiers in Immunology.2025;[Epub] CrossRef
Nanoencapsulation of Biotics: Feasibility to Enhance Stability and Delivery for Improved Gut Health
Pedro Brivaldo Viana da Silva, Thiécla Katiane Osvaldt Rosales, João Paulo Fabi
Pharmaceutics.2025; 17(9): 1180. CrossRef
Microbiota Modulation as an Approach to Prevent the Use of Antimicrobials Associated with Canine Atopic Dermatitis
Tânia Lagoa, Luís Martins, Maria Cristina Queiroga
Biomedicines.2025; 13(10): 2372. CrossRef
The Skin Histopathology of Pro- and Parabiotics in a Mouse Model of Atopic Dermatitis
Hun Hwan Kim, Se Hyo Jeong, Min Yeong Park, Pritam Bhagwan Bhosale, Abuyaseer Abusaliya, Jeong Doo Heo, Hyun Wook Kim, Je Kyung Seong, Tae Yang Kim, Jeong Woo Park, Byeong Soo Kim, Gon Sup Kim
Nutrients.2024; 16(17): 2903. CrossRef
Limosilactobacillus fermentum KBL674 Alleviates Vaginal Candidiasis
Sung Jae Jang, Eun Jung Jo, Cheonghoon Lee, Bo-Ram Cho, Yun Jeong Shin, Jun Soo Song, Woon-Ki Kim, Nanhee Lee, Hyungjin Lee, SungJun Park, GwangPyo Ko
Probiotics and Antimicrobial Proteins.2024;[Epub] CrossRef

Environmental Adaptation of Psychrophilic Bacteria Subtercola spp. Isolated from Various Cryospheric Habitats: Hanbyul Lee , Yong-Joon Cho , Ahnna Cho , Ok-Sun Kim; J. Microbiol. 2023;61(7):663-672. Published online August 24, 2023; DOI: https://doi.org/10.1007/s12275-023-00068-y

181 View
0 Download

Abstract PDF: Subtercola boreus K300T is a novel psychrophilic strain that was isolated from permanently cold groundwater in Finland and has also been found in several places in Antarctica including lake, soil, and rocks. We performed genomic and transcriptomic analyses of 5 strains from Antarctica and a type strain to understand their adaptation to different environments. Interestingly, the isolates from rocks showed a low growth rate and smaller genome size than strains from the other isolation sources (lake, soil, and groundwater). Based on these habitat-dependent characteristics, the strains could be classified into two ecotypes, which showed differences in energy production, signal transduction, and transcription in the clusters of orthologous groups of proteins (COGs) functional category. In addition, expression pattern changes revealed differences in metabolic processes, including uric acid metabolism, DNA repair, major facilitator superfamily (MFS) transporters, and xylose degradation, depending on the nutritional status of their habitats. These findings provide crucial insights into the environmental adaptation of bacteria, highlighting genetic diversity and regulatory mechanisms that enable them to thrive in the cryosphere.

Heterologous Production and Structure Determination of a New Lanthipeptide Sinosporapeptin Using a Cryptic Gene Cluster in an Actinobacterium Sinosporangium siamense: Keita Saito , Keiichiro Mukai , Issara Kaweewan , Hiroyuki Nakagawa , Takeshi Hosaka , Shinya Kodani; J. Microbiol. 2023;61(6):641-648. Published online June 12, 2023; DOI: https://doi.org/10.1007/s12275-023-00059-z

243 View
0 Download
6 Web of Science
3 Crossref

Abstract PDF

Lipolanthine is a subclass of lanthipeptide that has the modification of lipid moiety at the N-terminus. A cryptic biosynthetic gene cluster comprising four genes (sinA, sinKC, sinD, and sinE) involved in the biosynthesis of lipolanthine was identified in the genome of an actinobacterium Sinosporangium siamense. Heterologous coexpression of a precursor peptide coding gene sinA and lanthipeptide synthetase coding gene sinKC in the host Escherichia coli strain BL21(DE3) resulted in the synthesis of a new lanthipeptide, sinosporapeptin. It contained unusual amino acids, including one labionin and two dehydrobutyrine residues, as determined using NMR and MS analyses. Another coexpression experiment with two additional genes of decarboxylase (sinD) and N-acetyl transferase (sinE) resulted in the production of a lipolanthine-like modified sinosporapeptin.

Citations

Citations to this article as recorded by

Heterologous biosynthesis of myxobacterial lanthipeptides melittapeptins
Issara Kaweewan, Keiichiro Mukai, Pratchaya Rukthanapitak, Hiroyuki Nakagawa, Takeshi Hosaka, Shinya Kodani
Applied Microbiology and Biotechnology.2024;[Epub] CrossRef
Facile Method for Determining Lanthipeptide Stereochemistry
Youran Luo, Shuyun Xu, Autumn M. Frerk, Wilfred A. van der Donk
Analytical Chemistry.2024; 96(4): 1767. CrossRef
Antimicrobial Peptides Derived from Bacteria: Classification, Sources, and Mechanism of Action against Multidrug-Resistant Bacteria
Raynichka Mihaylova-Garnizova, Slavena Davidova, Yordan Hodzhev, Galina Satchanska
International Journal of Molecular Sciences.2024; 25(19): 10788. CrossRef

Identification and Characterization of HEPN‑MNT Type II TA System from Methanothermobacter thermautotrophicus ΔH: Wonho Choi , Anoth Maharjan , Hae Gang Im , Ji-Young Park , Jong-Tae Park , Jung-Ho Park; J. Microbiol. 2023;61(4):411-421. Published online April 18, 2023; DOI: https://doi.org/10.1007/s12275-023-00041-9

262 View
0 Download
1 Web of Science
1 Crossref

Abstract PDF

Toxin-antitoxin (TA) systems are widespread in bacteria and archaea plasmids and genomes to regulate DNA replication, gene transcr!ption, or protein translation. Higher eukaryotic and prokaryotic nucleotide-binding (HEPN) and minimal nucleotidyltransferase (MNT) domains are prevalent in prokaryotic genomes and constitute TA pairs. However, three gene pairs (MTH304/305, 408/409, and 463/464) of Methanothermobacter thermautotropicus ΔH HEPN-MNT family have not been studied as TA systems. Among these candidates, our study characterizes the MTH463/MTH464 TA system. MTH463 expression inhibited Escherichia coli growth, whereas MTH464 did not and blocked MTH463 instead. Using site-directed MTH463 mutagenesis, we determined that amino acids R99G, H104A, and Y106A from the R[ɸX]4-6H motif are involved with MTH463 cell toxicity. Furthermore, we established that purified MTH463 could degrade MS2 phage RNA, whereas purified MTH464 neutralized MTH463 activity in vitro. Our results indicate that the endonuclease toxin MTH463 (encoding a HEPN domain) and its cognate antitoxin MTH464 (encoding the MNT domain) may act as a type II TA system in M. thermautotropicus ΔH. This study provides initial and essential information studying TA system functions, primarily archaea HEPN-MNT family.

Citations

Citations to this article as recorded by

Extensive Genomic Rearrangement of Catalase-Less Cyanobloom-Forming Microcystis aeruginosa in Freshwater Ecosystems
Minkyung Kim, Jaejoon Jung, Wonjae Kim, Yerim Park, Che Ok Jeon, Woojun Park
Journal of Microbiology.2024; 62(11): 933. CrossRef

Vibrio vulnificus PlpA facilitates necrotic host cell death induced by the pore forming MARTX toxin: Changyi Cho , Sanghyeon Choi , Myung Hee Kim , Byoung Sik Kim; J. Microbiol. 2022;60(2):224-233. Published online February 1, 2022; DOI: https://doi.org/10.1007/s12275-022-1448-x

281 View
0 Download
11 Web of Science
9 Crossref

Abstract PDF

Opportunistic pathogen Vibrio vulnificus causes severe systemic infection in humans with high mortality. Although multiple exotoxins have been characterized in V. vulnificus, their interactions and potential synergistic roles in pathogen-induced host cell death have not been investigated previously. By employing a series of multiple exotoxin deletion mutants, we investigated whether specific exotoxins of the pathogen functioned together to achieve severe and rapid necrotic cell death. Human epithelial cells treated with V. vulnificus with a plpA deletion background exhibited an unusually prolonged cell blebbing, suggesting the importance of PlpA, a phospholipase A2, in rapid necrotic cell death by this pathogen. Additional deletion of the rtxA gene encoding the multifunctional autoprocessing repeats-in-toxin (MARTX) toxin did not result in necrotic cell blebs. However, if the rtxA gene was engineered to produce an effector-free MARTX toxin, the cell blebbing was observed, indicating that the pore forming activity of the MARTX toxin is sufficient, but the MARTX toxin effector domains are not necessary, for the blebbing. When a recombinant PlpA was treated on the blebbed cells, the blebs were completely disrupted. Consistent with this, MARTX toxin-pendent rapid release of cytosolic lactate dehydrogenase was significantly delayed in the plpA deletion background. Mutations in other exotoxins such as elastase, cytolysin/hemolysin, and/or extracellular metalloprotease did not affect the bleb formation or disruption. Together, these findings indicate that the pore forming MARTX toxin and the phospholipase A2, PlpA, cooperate sequentially to achieve rapid necrotic cell death by inducing cell blebbing and disrupting the blebs, respectively.

Citations

Citations to this article as recorded by

Genome-wide phenotypic profiling of transcription factors and identification of novel targets to control the virulence of Vibrio vulnificus
Dayoung Sung, Garam Choi, Minji Ahn, Hokyung Byun, Tae Young Kim, Hojun Lee, Zee-Won Lee, Ji Yong Park, Young Hyun Jung, Ho Jae Han, Sang Ho Choi
Nucleic Acids Research.2025;[Epub] CrossRef
Differential expressed genes (DEGs) and differential alternative splicing genes (DASs) revealed the common pathologic mechanism of three bacterial pathogens to American eels (Anguilla rostrata)
Zihao Chen, Guanghua Sun, Songlin Guo
Aquaculture Reports.2025; 40: 102632. CrossRef
Implication of environmental factors on the pathogenicity of Vibrio vulnificus: Insights into gene activation and disease outbreak
Aswathi Bharathan, Yaser Arafath, Aifa Fathima, Saqib Hassan, Prabhakar Singh, George Seghal Kiran, Joseph Selvin
Microbial Pathogenesis.2025; 204: 107591. CrossRef
Non-Repeat Segment 1 in Vibrio vulnificus MARTX toxin, which binds to biantennary N-glycans, is essential for host cell blebbing but dispensable for effector translocation
Jieun Lee, Yunjeong Kim, Byoung Sik Kim
Microbiological Research.2025; 295: 128108. CrossRef
Vibrio-infecting bacteriophages and their potential to control biofilm
Ana Cevallos-Urena, Jeong Yeon Kim, Byoung Sik Kim
Food Science and Biotechnology.2023; 32(12): 1719. CrossRef
Pathogenic Mechanism of Vibrio Vulnificus Infection
Kun Lu, Yang Li, Rui Chen, Hua Yang, Yong Wang, Wei Xiong, Fang Xu, Qijun Yuan, Haihui Liang, Xian Xiao, Renqiang Huang, Zhipeng Chen, Chunou Tian, Songqing Wang
Future Microbiology.2023; 18(6): 373. CrossRef
Functional conservation of specialized ribosomes bearing genome-encoded variant rRNAs in Vibrio species
Younkyung Choi, Eunkyoung Shin, Minho Lee, Ji-Hyun Yeom, Kangseok Lee, Bashir Sajo Mienda
PLOS ONE.2023; 18(12): e0289072. CrossRef
Complex regulatory networks of virulence factors in Vibrio vulnificus
Garam Choi, Sang Ho Choi
Trends in Microbiology.2022; 30(12): 1205. CrossRef
MARTX toxin of Vibrio vulnificus induces RBC phosphatidylserine exposure that can contribute to thrombosis
Han Young Chung, Yiying Bian, Kyung-Min Lim, Byoung Sik Kim, Sang Ho Choi
Nature Communications.2022;[Epub] CrossRef

Vagococcus zengguangii sp. nov., isolated from yak faeces: Yajun Ge , Dong Jin , Xin-He Lai , Jing Yang , Shan Lu , Ying Huang , Han Zheng , Xiaoyan Zhang , Jianguo Xu; J. Microbiol. 2021;59(1):1-9. Published online December 23, 2020; DOI: https://doi.org/10.1007/s12275-021-0406-3

228 View
0 Download
3 Web of Science
2 Crossref

Abstract PDF

Two unknown Gram-stain-positive, catalase- and oxidasenegative, non-motile, and coccus-shaped bacteria, designated MN-17T and MN-09, were isolated from yaks faeces (Bos grunniens) in the Qinghai-Tibet Plateau of China. 16S rRNA gene sequence-based comparative analyses revealed that the two strains were grouped within the genus Vagococcus, displaying the highest similarity with Vagococcus xieshaowenii CGMCC 1.16436T (98.6%) and Vagococcus elongatus CCUG 51432T (96.4%). Both strains grew optimally at 37°C and pH 7.0 in the presence of 0.5% (w/v) NaCl. The complete genome of MN-17T comprises 2,085 putative genes with a total of 2,190,262 bp and an average G + C content of 36.7 mol%. The major fatty acids were C16:0 (31.2%), C14:0 (28.5%), and C18:1ω9c (13.0%); the predominant respiratory quinone was MK-7 (68.8%); the peptidoglycan type was A4α(L-Lys-DAsp); and the major polar lipid was diphosphatidylglycerol. Together, these supported the affiliation of strain MN-17T to the genus Vagococcus. In silico DNA-DNA hybridization and the average nucleotide identity values between MN-17T and all recognized species in the genus were 21.6–26.1% and 70.7–83.0%, respectively. MN-17T produced acid from D-cellobiose, D-fructose, glycerol, D-glucose, N-acetyl-glucosamine, gentiobiose, D-mannose, D-maltose, D-ribose, Dsaccharose, salicin, D-trehalose, and D-xylose. These results distinguished MN-17T and MN-09 from closely related species in Vagococcus. Thus, we propose that strains MN-17T and MN-09 represent a novel species in the genus Vagococcus, with the name Vagococcus zengguangii sp. The type strain is MN-17T (= CGMCC 1.16726T = GDMCC 1.1589T = JCM 33478T).

Citations

Citations to this article as recorded by

Vagococcus proximus sp. nov. and Vagococcus intermedius sp. nov., originating from modified atmosphere packaged broiler meat
Per Johansson, Elina Jääskeläinen, Elina Säde, Johanna Björkroth
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef
Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp. nov., a novel halotolerant actinomycete isolated from bat feces
Yuyuan Huang, Lingzhi Dong, Jian Gong, Jing Yang, Shan Lu, Xin-He Lai, Dong Jin, Qianni Huang, Ji Pu, Liyun Liu, Jianguo Xu
Journal of Microbiology.2022; 60(10): 977. CrossRef

Analyses of DNA double-strand break repair pathways in tandem arrays of HXT genes of Saccharomyces cerevisiae: Ju-Hee Choi , Ye-Seul Lim , Min-Ku Kim , Sung-Ho Bae; J. Microbiol. 2020;58(11):957-966. Published online October 30, 2020; DOI: https://doi.org/10.1007/s12275-020-0461-1

245 View
0 Download
4 Web of Science
4 Crossref

Abstract PDF

Eukaryotic genomes contain numerous homologous repeat sequences including redundant genes with divergent homology that can be potential recombination targets. Recombination between divergent sequences is rare but poses a substantial threat to genome stability. The hexose transporter (HXT) gene family shares high sequence similarities at both protein and DNA levels, and some members are placed close together in tandem arrays. In this study, we show that spontaneous interstitial deletions occur at significantly high rates in HXT gene clusters, resulting in chimeric HXT sequences that contain a single junction point. We also observed that DNA double-strand breaks created between HXT genes produce primarily interstitial deletions, whereas internal cleavage of the HXT gene resulted in gene conversions as well as deletion products. Interestingly, interstitial deletions were less constrained by sequence divergence than gene conversion. Moreover, recombination-defective mutations differentially affected the survival frequency. Mutations that impair single-strand annealing (SSA) pathway greatly reduced the survival frequency by 10–1,000-fold, whereas disruption of Rad51-dependent homologous recombination exhibited only modest reduction. Our results indicate that recombination in the tandemly repeated HXT genes occurs primarily via SSA pathway.

Citations

Citations to this article as recorded by

Deletion of IRC19 Causes Defects in DNA Double-Strand Break Repair Pathways in Saccharomyces cerevisiae
Ju-Hee Choi, Oyungoo Bayarmagnai, Sung-Ho Bae
Journal of Microbiology.2024; 62(9): 749. CrossRef
A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris
Xiang Wang, Yi Li, Zhehao Jin, Xiangjian Liu, Xiang Gao, Shuyuan Guo, Tao Yu
Synthetic and Systems Biotechnology.2023; 8(3): 445. CrossRef
Enhancing Homologous Recombination Efficiency in Pichia pastoris for Multiplex Genome Integration Using Short Homology Arms
Jucan Gao, Cuifang Ye, Jintao Cheng, Lihong Jiang, Xinghao Yuan, Jiazhang Lian
ACS Synthetic Biology.2022; 11(2): 547. CrossRef
Effects of the loss of mismatch repair genes on single-strand annealing between divergent sequences in Saccharomyces cerevisiae
Ye-Seul Lim, Ju-Hee Choi, Kyu-Jin Ahn, Min-Ku Kim, Sung-Ho Bae
Journal of Microbiology.2021; 59(4): 401. CrossRef

Iron interferes with quorum sensing-mediated cooperation in Pseudomonas aeruginosa by affecting the expression of ppyR and mexT, in addition to rhlR: Feng Sun , Na Li , Lijia Wang , Huajun Feng , Dongsheng Shen , Meizhen Wang; J. Microbiol. 2020;58(11):938-944. Published online October 30, 2020; DOI: https://doi.org/10.1007/s12275-020-0264-4

259 View
0 Download
4 Web of Science
4 Crossref

Abstract PDF

The stabilization of quorum sensing (QS) is vital for bacterial survival in various environments. Although the mechanisms of QS stabilization in certain conditions have been well studied, the impact of environmental factors has received much less attention. In this study, we show that the supplementation of 25 μM iron in competition experiments and 50 μM in evolution experiments to casein growth cultures significantly increased the possibility of population collapse by affecting elastase production. However, the expression of lasI and lasR remained constant regardless of iron concentration and hence this effect was not through interference with the LasIR circuit, which mainly regulates the secretion of elastase in Pseudomonas aeruginosa. However, the expression of rhlR was significantly inhibited by iron treatment, which could affect the production of elastase. Further, based on both reverse transcription quantitative polymerase chain reaction and gene knock-out assays, we show that iron inhibits the transcription of ppyR and enhances the expression of mexT, both of which decrease elastase production and correspondingly interfere with QS stabilization. Our findings show that environmental factors can affect the genes of QS circuits, interfering with QS stabilization. These findings are not only beneficial in understanding the mechanistic effect of iron on QS stabilization, but also demonstrate the complexity of QS stabilization by linking non-QS-related genes with QS traits.

Citations

Citations to this article as recorded by

Effect of LuxS/AI-2-mediated quorum sensing system on bacteriocin production of Lactobacillus plantarum NMD-17
Li-Li Man, Dian-Jun Xiang
Folia Microbiologica.2023; 68(6): 855. CrossRef
PtsN in Pseudomonas aeruginosa Is Phosphorylated by Redundant Upstream Proteins and Impacts Virulence-Related Genes
Simon A. M. Underhill, Somalisa Pan, Mary Erdmann, Matthew T. Cabeen, Joseph Bondy-Denomy
Journal of Bacteriology.2023;[Epub] CrossRef
The immune responses to different Uropathogens call individual interventions for bladder infection
Linlong Li, Yangyang Li, Jiali Yang, Xiang Xie, Huan Chen
Frontiers in Immunology.2022;[Epub] CrossRef
Design of Polymeric Thin Films to Direct Microbial Biofilm Growth, Virulence, and Metabolism
Trevor Franklin, Yinan Wu, Jiayan Lang, Sijin Li, Rong Yang
Biomacromolecules.2021; 22(12): 4933. CrossRef

Anti-inflammatory and anti-oxidative effect of Korean propolis on Helicobacter pylori-induced gastric damage in vitro: Moon-Young Song , Da-Young Lee , Eun-Hee Kim; J. Microbiol. 2020;58(10):878-885. Published online September 2, 2020; DOI: https://doi.org/10.1007/s12275-020-0277-z

377 View
0 Download
32 Web of Science
31 Crossref

Abstract PDF

Helicobacter pylori, present in the stomach lining, is a Gramnegative bacterium that causes various gastrointestinal diseases, including gastritis and peptic ulcers. Propolis is a natural resinous substance collected from a variety of plants, and contains several natural bioactive substances. The aim of this study was to investigate the anti-inflammatory and antioxidative effects of Korean propolis on H. pylori-induced damage in the human adenocarcinoma gastric cell line. The propolis used in this study was obtained from the Korea Beekeeping Association in South Korea. The expression of pro-inflammatory interleukins (ILs), such as IL-8, IL-12, IL-1β, tumor necrosis factor alpha, cyclooxygenase-2, and inducible nitric oxide synthase, which was increased after H. pylori infection, significantly decreased in a dose-dependent manner upon pretreatment with Korean propolis, because of the suppression of mitogen-activated protein kinases and nuclear factor κB pathway. The anti-oxidative activity of propolis was assessed using the 2,2-diphenyl-1-picrylhydrazyl hydrate free radical assay. Korean propolis showed significant anti-oxidative effects via reactive oxygen species scavenging. In addition, pretreatment with Korean propolis upregulated the expression of anti-oxidant enzymes through Nrf2 signaling activation. These findings indicate that the use of Korean propolis, which has anti-inflammatory and anti-oxidative effects, can be promising for the prevention of H. pylori-induced gastric damage.

Citations

Citations to this article as recorded by

Network Pharmacology and Experimental Validation Reveal Therapeutic Potential of Propolis in UV-Induced Allergic Dermatitis
Liyuan Cheng, Jie Wang, Yicong Wang, Jingjing Li, Wenchao Yang
Foods.2025; 14(6): 996. CrossRef
Propolis Stands out as a Multifaceted Natural Product: Meta-Analysis on Its Sources, Bioactivities, Applications, and Future Perspectives
Ahmed Sabri Ayad, Samia Benchaabane, Tarek Daas, Guy Smagghe, Wahida Loucif-Ayad
Life.2025; 15(5): 764. CrossRef
Bee products in the fight against Helicobacter pylori and molecular interactions
Hesham R. El-Seedi, Mohamed S. Refaey, Aida A. Abd El-Wahed, Aida Albadawy, Sercan Karav, Shaden H. El-Seedi, Guiguang Cheng, Mohamed F. Salem, Lianliang Liu, Jie Tang, Tariq Z. Abolibda, Xiaobo Zou, Zhiming Guo, Shaden A.M. Khalifa
Microbial Pathogenesis.2025; 205: 107707. CrossRef
Potential Efficacy of Propolis in Treating Helicobacter pylori Infection and Its Mechanisms of Action
Haitao Nie, Qing Li, Keke Zhao, Wen Li, Cuiping Zhang, Xiasen Jiang
Nutrients.2025; 17(17): 2803. CrossRef
Propolis as an autophagy modulator in relation to its roles in redox balance and inflammation regulation
R. Lesmana, S. Tandean, A. Christoper, A.A. Suwantika, N. Wathoni, R. Abdulah, J. Fearnley, V. Bankova, F. Zulhendri
Biomedicine & Pharmacotherapy.2024; 175: 116745. CrossRef
Exploring the Prospective Role of Propolis in Modifying Aging Hallmarks
Carla Scorza, Valeria Goncalves, Josef Finsterer, Fúlvio Scorza, Fernando Fonseca
Cells.2024; 13(5): 390. CrossRef
Development of a chitosan/propolis-based polymeric system: Characterization, biocompatibility, and modulation of transcription factor expression
Raquel Velázquez-Rodríguez, Amaury Pozos-Guillén, Martha Gabriela Chuc-Gamboa, Juan Valerio Cauich-Rodríguez, Héctor Flores-Reyes, Francisco Javier Tejeda-Nava, Fernando Javier Aguilar-Perez, Diana Maria Escobar Garcia
Journal of Bioactive and Compatible Polymers.2024; 39(5): 329. CrossRef
Therapeutic potential of propolis in alleviating inflammatory response and promoting wound healing in skin burn
Christian Oktavianus Manginstar, Trina Ekawati Tallei, Nurdjannah Jane Niode, Christina Leta Salaki, Sofia Safitri Hessel
Phytotherapy Research.2024; 38(2): 856. CrossRef
A review for non-antibiotic treatment of Helicobacter pylori: new insight
Neda Shadvar, Sousan Akrami, Seyyed-Mohammad-Amin Mousavi Sagharchi, Rafee Habib Askandar, Alireza Merati, Masoomeh Aghayari, Nikki Kaviani, Hamed Afkhami, Mojtaba Kashfi
Frontiers in Microbiology.2024;[Epub] CrossRef
Wound Healing, Anti-Inflammatory and Anti-Oxidant Activities, and Chemical Composition of Korean Propolis from Different Sources
Aman Dekebo, Chalshisa Geba, Daniel Bisrat, Jin Boo Jeong, Chuleui Jung
International Journal of Molecular Sciences.2024; 25(21): 11352. CrossRef
Effects of oxidative stress regulation in inflammation-associated gastric cancer progression treated using traditional Chinese medicines: A review
Bo Chen, Xinqian Dong, Jinlong Zhang, Wei Wang, Yujiao Song, Xitong Sun, Kangning Zhao, Zhen Sun
Medicine.2023; 102(46): e36157. CrossRef
Emodin Attenuates Inflammasome Activation Induced by Helicobacter pylori Infection through Inhibition of VacA Translocation
Thach Phan Van, Anh Duy Do
Microbiology and Biotechnology Letters.2023; 51(4): 507. CrossRef
Correlation between Chemical Profile of Georgian Propolis Extracts and Their Activity against Helicobacter pylori
Jarosław Widelski, Piotr Okińczyc, Katarzyna Suśniak, Anna Malm, Anna Bozhadze, Malkhaz Jokhadze, Izabela Korona-Głowniak
Molecules.2023; 28(3): 1374. CrossRef
Therapeutic effect of propolis nanoparticles on wound healing
Juan Yang, Yingjuan He, Sha Nan, Juan Li, Anjuan Pi, Lele Yan, Jinshan Xu, Yuhui Hao
Journal of Drug Delivery Science and Technology.2023; 82: 104284. CrossRef
Recent Update on the Anti-Inflammatory Activities of Propolis
Felix Zulhendri, Ronny Lesmana, Steven Tandean, Andreas Christoper, Kavita Chandrasekaran, Ilham Irsyam, Auliya A. Suwantika, Rizky Abdulah, Nasrul Wathoni
Molecules.2022; 27(23): 8473. CrossRef
Construction of a Cuprotosis-Related Gene-Based Model to Improve the Prognostic Evaluation of Patients with Gastric Cancer
Chunyan Han, Kai Zhang, XinKai Mo, Fu Wang
Journal of Immunology Research.2022; 2022: 1. CrossRef
Honey Bee Products: Preclinical and Clinical Studies of Their Anti-inflammatory and Immunomodulatory Properties
Hesham R. El-Seedi, Nehal Eid, Aida A. Abd El-Wahed, Mostafa E. Rateb, Hanan S. Afifi, Ahmed F. Algethami, Chao Zhao, Yahya Al Naggar, Sultan M. Alsharif, Haroon Elrasheid Tahir, Baojun Xu, Kai Wang, Shaden A. M. Khalifa
Frontiers in Nutrition.2022;[Epub] CrossRef
Plant-Based Polyphenols: Anti-Helicobacter pylori Effect and Improvement of Gut Microbiota
María Guerra-Valle, Patricio Orellana-Palma, Guillermo Petzold
Antioxidants.2022; 11(1): 109. CrossRef
DETERMINATION OF ANTI-UREASE ACTIVITY OF PROPOLIS FROM MARMARA REGION OF TURKEY
Zehra Can, Yakup Kara, Sevgi Kolaylı, İbrahim Çakmak
Uludağ Arıcılık Dergisi.2022; 22(1): 25. CrossRef
Nrf2 Downregulation Contributes to Epithelial-to-Mesenchymal Transition in Helicobacter pylori-Infected Cells
Sarah Bacon, Lornella Seeneevassen, Alison Fratacci, Faustine Rose, Camille Tiffon, Elodie Sifré, Maria M. Haykal, Maya M. Moubarak, Astrid Ducournau, Lucie Bruhl, Stéphane Claverol, Caroline Tokarski, Alina-Roxani Gouloumi, Ioannis S. Pateras, Thomas Dau
Cancers.2022; 14(17): 4316. CrossRef
Anti-Inflammatory Effect of Korean Propolis on Helicobacter pylori-Infected Gastric Mucosal Injury Mice Model
Moon-Young Song, Da-Young Lee, Young-Min Han, Eun-Hee Kim
Nutrients.2022; 14(21): 4644. CrossRef
Antifungal Properties of Chemically Defined Propolis from Various Geographical Regions
Marcin Ożarowski, Tomasz M. Karpiński, Rahat Alam, Małgorzata Łochyńska
Microorganisms.2022; 10(2): 364. CrossRef
The Antimicrobial Properties of Poplar and Aspen–Poplar Propolises and Their Active Components against Selected Microorganisms, including Helicobacter pylori
Jarosław Widelski, Piotr Okińczyc, Emil Paluch, Tomasz Mroczek, Jakub Szperlik, Magdalena Żuk, Zbigniew Sroka, Zuriyadda Sakipova, Ioanna Chinou, Krystyna Skalicka-Woźniak, Anna Malm, Izabela Korona-Głowniak
Pathogens.2022; 11(2): 191. CrossRef
Research Progress on Therapeutic Effect and Mechanism of Propolis on Wound Healing
Juan Yang, Anjuan Pi, Lele Yan, Juan Li, Sha Nan, Jing Zhang, Yuhui Hao, Juraj Majtan
Evidence-Based Complementary and Alternative Medicine.2022; 2022: 1. CrossRef
"GREEN SYNTHESIS OF NANO-SILVER/SODIUM ALGINATE/CARBOXYMETHYL XANTHAN GUM HYDROGEL AND EVALUATION OF ITS ANTI-INFLAMMATORY AND ANTI-Helicobacter pylori ACTIVITY "
NOURA Y. ELMEHBAD, NADIA A. MOHAMED, NAHED A. ABD EL-GHANY, MARWA M. ABDEL-AZIZ
Cellulose Chemistry and Technology.2022; 56(9-10): 983. CrossRef
Propolis-Loaded Chitosan Nanoparticles for the Treatment of Bacterial Diarrhea: In Vitro and In Vivo Evaluation
Jie Qiao, Jun Yang, Saman Jalili
Journal of Biomedical Nanotechnology.2022; 18(7): 1826. CrossRef
Steamed Ginger Extract Exerts Anti-inflammatory Effects in Helicobacter pylori-infected Gastric Epithelial Cells through Inhibition of NF-κB
Moon-Young Song, Da-Young Lee, Sang-Yong Park, Seul-A Seo, Jeong-Seung Hwang, Soo-Hyeon Heo, Eun-Hee Kim
Journal of Cancer Prevention.2021; 26(4): 289. CrossRef
Short‐term efficacy of a gel containing propolis extract, nanovitamin C and nanovitamin E on peri‐implant mucositis: A double‐blind, randomized, clinical trial
José González‐Serrano, Rosa María López‐Pintor, Julia Serrano, Jesús Torres, Gonzalo Hernández, Mariano Sanz
Journal of Periodontal Research.2021; 56(5): 897. CrossRef
Experimental Evidence for Therapeutic Potentials of Propolis
Priyanshu Bhargava, Debajit Mahanta, Ashish Kaul, Yoshiyuki Ishida, Keiji Terao, Renu Wadhwa, Sunil C. Kaul
Nutrients.2021; 13(8): 2528. CrossRef
Propolis and Its Gastroprotective Effects on NSAID-Induced Gastric Ulcer Disease: A Systematic Review
Porfirio Alonso Ruiz-Hurtado, Leticia Garduño-Siciliano, Pilar Domínguez-Verano, Daniela Balderas-Cordero, Gustavo Gorgua-Jiménez, Octavio Canales-Álvarez, María Margarita Canales-Martínez, Marco Aurelio Rodríguez-Monroy
Nutrients.2021; 13(9): 3169. CrossRef
Molecular Insights into the Antistress Potentials of Brazilian Green Propolis Extract and Its Constituent Artepillin C
Ashish Kaul, Raviprasad Kuthethur, Yoshiyuki Ishida, Keiji Terao, Renu Wadhwa, Sunil C. Kaul
Molecules.2021; 27(1): 80. CrossRef

Caspase-3 inhibitor inhibits enterovirus D68 production: Wenbo Huo , Jinghua Yu , Chunyu Liu , Ting Wu , Yue Wang , Xiangling Meng , Fengmei Song , Shuxia Zhang , Ying Su , Yumeng Liu , Jinming Liu , Xiaoyan Yu , Shucheng Hua; J. Microbiol. 2020;58(9):812-820. Published online September 1, 2020; DOI: https://doi.org/10.1007/s12275-020-0241-y

256 View
0 Download
8 Web of Science
8 Crossref

Abstract PDF

Enterovirus D68 (EVD68) is an emerging pathogen that recently caused a large worldwide outbreak of severe respiratory disease in children. However, the relationship between EVD68 and host cells remains unclear. Caspases are involved in cell death, immune response, and even viral production. We found that caspase-3 was activated during EVD68 replication to induce apoptosis. Caspase-3 inhibitor (Z-DEVDFMK) inhibited viral production, protected host cells from the cytopathic effects of EVD68 infection, and prevented EVD68 from regulating the host cell cycle at G0/G1. Meanwhile, caspase-3 activator (PAC-1) increased EVD68 production. EVD68 infection therefore activates caspase-3 for virus production. This knowledge provides a potential direction for the prevention and treatment of disease related to EVD68.

Citations

Citations to this article as recorded by

Non-Polio Enterovirus Inhibitors: Scaffolds, Targets, and Potency─What’s New?
Hugo Fernando Georges Roux, Franck Touret, Pascal Rathelot, Pietro Sciò, Antonio Coluccia, Patrice Vanelle, Manon Roche
ACS Infectious Diseases.2025; 11(1): 21. CrossRef
Mode of cell death in the penile cavernous tissue of type 1 diabetes mellitus rats
Jing Li, Qilan Jiang, Jun Jiang, Rui Jiang
The Journal of Sexual Medicine.2024; 21(8): 652. CrossRef
MDA5 Enhances Invasive Candida albicans Infection by Regulating Macrophage Apoptosis and Phagocytosis/Killing Functions
Yayun Chen, Qian Jiang, Furong Qing, Junxia Xue, Qiuxiang Xiao, Wenji He, Lina Sui, Zhiping Liu
Inflammation.2024; 47(1): 191. CrossRef
Caspase-8 activation regulates enterovirus D68 infection-induced inflammatory response and cell death
Yuanyuan Zhou, Chongtao Zhang, Yuhan Zhang, Fei Li, Jun Shen
Biosafety and Health.2024; 6(3): 171. CrossRef
Enterovirus D68 Infection Induces TDP-43 Cleavage, Aggregation, and Neurotoxicity
Lili Zhang, Jiaxin Yang, Huili Li, Zhe Zhang, Zhilin Ji, Lirong Zhao, Wei Wei, Rebecca Ellis Dutch
Journal of Virology.2023;[Epub] CrossRef
Inhibitory effect of tanshinone IIA, resveratrol and silibinin on enterovirus 68 production through inhibiting ATM and DNA-PK pathway
Ying Su, Ting Wu, Xiao-Yan Yu, Wen-Bo Huo, Shao-Hua Wang, Chen Huan, Yu-Meng Liu, Jin-Ming Liu, Min-Na Cui, Xin-Hua Li, Jing-Hua Yu
Phytomedicine.2022; 99: 153977. CrossRef
Urolithin A inhibits enterovirus 71 replication and promotes autophagy and apoptosis of infected cells in vitro
Shengyu Wang, Junhua Qiao, Yaping Chen, Langfei Tian, Xin Sun
Archives of Virology.2022; 167(10): 1989. CrossRef
Mst1/2-ALK promotes NLRP3 inflammasome activation and cell apoptosis during Listeria monocytogenes infection
Aijiao Gao, Huixin Tang, Qian Zhang, Ruiqing Liu, Lin Wang, Yashan Liu, Zhi Qi, Yanna Shen
Journal of Microbiology.2021; 59(7): 681. CrossRef

First
Prev
Page of 4
Next
Last

Search